{"doi":"10.1016/j.crmeth.2024.100796","title":"Development of an efficient, effective, and economical technology for proteome analysis","abstract":"We present an efficient, effective, and economical approach, named E3technology, for proteomics sample preparation. By immobilizing silica microparticles into the polytetrafluoroethylene matrix, we develop a robust membrane medium, which could serve as a reliable platform to generate proteomics-friendly samples in a rapid and low-cost fashion. We benchmark its performance using different formats and demonstrate them with a variety of sample types of varied complexity, quantity, and volume. Our data suggest that E3technology provides proteome-wide identification and quantitation performance equivalent or superior to many existing methods. We further propose an enhanced single-vessel approach, named E4technology, which performs on-filter in-cell digestion with minimal sample loss and high sensitivity, enabling low-input and low-cell proteomics. Lastly, we utilized the above technologies to investigate RNA-binding proteins and profile the intact bacterial cell proteome.","journal":"Cell Reports Methods","year":2024,"id":422606,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9551,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":965363,"name":"Ha T. Le","orcid":null,"position":1,"is_corresponding":false},{"id":887144,"name":"Ahmed Abdelgawad","orcid":"0000-0002-8175-639X","position":2,"is_corresponding":false},{"id":1217581,"name":"Canyuan Yang","orcid":null,"position":3,"is_corresponding":false},{"id":1216911,"name":"Guotao Lu","orcid":"0000-0002-8325-0405","position":4,"is_corresponding":false},{"id":664698,"name":"Jessica L. Keffer","orcid":"0000-0002-0302-3588","position":5,"is_corresponding":false},{"id":235522,"name":"Xiaohui Zhang","orcid":"0000-0003-0245-6792","position":6,"is_corresponding":false},{"id":670362,"name":"Zhihao Zhuang","orcid":"0000-0001-5047-9222","position":7,"is_corresponding":false},{"id":485672,"name":"Papa Nii Asare‐Okai","orcid":null,"position":8,"is_corresponding":false},{"id":86954,"name":"Clara S. Chan","orcid":"0000-0003-1810-4994","position":9,"is_corresponding":false},{"id":255907,"name":"Mona Batish","orcid":"0000-0001-9039-6149","position":10,"is_corresponding":false},{"id":11782,"name":"Yanbao Yu","orcid":"0000-0003-2994-1974","position":11,"is_corresponding":false},{"id":1216910,"name":"Katherine Martin","orcid":"0000-0001-9453-9573","position":0,"is_corresponding":true}],"reference_count":85,"raw_metadata":null,"created_at":"2026-07-19T01:57:51.642883Z","pmid":"38866007","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}