{"doi":"10.1016/j.crmeth.2023.100690","title":"Enhanced detection of antigen-specific T cells by a multiplexed AIM assay","abstract":"Broadly applicable methods to identify and characterize antigen-specific CD4+ and CD8+ T cells are key to immunology research, including studies of vaccine responses and immunity to infectious diseases. We developed a multiplexed activation-induced marker (AIM) assay that presents several advantages compared to single pairs of AIMs. The simultaneous measurement of four AIMs (CD69, 4-1BB, OX40, and CD40L) creates six AIM pairs that define CD4+ T cell populations with partial and variable overlap. When combined in an AND/OR Boolean gating strategy for analysis, this approach enhances CD4+ T cell detection compared to any single AIM pair, while CD8+ T cells are dominated by CD69/4-1BB co-expression. Supervised and unsupervised clustering analyses show differential expression of the AIMs in defined T helper lineages and that multiplexing mitigates phenotypic biases. Paired and unpaired comparisons of responses to infections (HIV and cytomegalovirus [CMV]) and vaccination (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) validate the robustness and versatility of the method.","journal":"Cell Reports Methods","year":2024,"id":420348,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9583,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347020,"name":"Gérémy Sannier","orcid":"0000-0001-7956-4463","position":1,"is_corresponding":false},{"id":896275,"name":"Alexandre Nicolas","orcid":"0000-0002-8953-3924","position":2,"is_corresponding":false},{"id":623260,"name":"Manon Nayrac","orcid":"0000-0001-5717-6232","position":3,"is_corresponding":false},{"id":349169,"name":"Gloria-Gabrielle Delgado","orcid":null,"position":4,"is_corresponding":false},{"id":1211741,"name":"Rose Cloutier","orcid":null,"position":5,"is_corresponding":false},{"id":347021,"name":"Nathalie Brassard","orcid":"0000-0002-4320-9416","position":6,"is_corresponding":false},{"id":843371,"name":"Mélanie Laporte","orcid":null,"position":7,"is_corresponding":false},{"id":1211742,"name":"Mélina Duchesne","orcid":null,"position":8,"is_corresponding":false},{"id":1211743,"name":"Alina Maria Sreng Flores","orcid":null,"position":9,"is_corresponding":false},{"id":241588,"name":"Andrés Finzi","orcid":"0000-0002-4992-5288","position":10,"is_corresponding":false},{"id":623261,"name":"Olivier Tastet","orcid":"0000-0001-5694-6266","position":11,"is_corresponding":false},{"id":254285,"name":"Mathieu Dubé","orcid":"0000-0002-5522-9019","position":12,"is_corresponding":false},{"id":254288,"name":"Daniel E. Kaufmann","orcid":"0000-0003-4467-136X","position":13,"is_corresponding":false},{"id":1211255,"name":"Audrée Lemieux","orcid":"0000-0002-5150-8010","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-19T01:57:25.777459Z","pmid":"38228152","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}