{"doi":"10.1016/j.crmeth.2023.100595","title":"Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation","abstract":"Imaging mass cytometry (IMC) is a powerful technique capable of detecting over 30 markers on a single slide. It has been increasingly used for single-cell-based spatial phenotyping in a wide range of samples. However, it only acquires a rectangle field of view (FOV) with a relatively small size and low image resolution, which hinders downstream analysis. Here, we reported a highly practical dual-modality imaging method that combines high-resolution immunofluorescence (IF) and high-dimensional IMC on the same tissue slide. Our computational pipeline uses the whole-slide image (WSI) of IF as a spatial reference and integrates small-FOV IMC into a WSI of IMC. The high-resolution IF images enable accurate single-cell segmentation to extract robust high-dimensional IMC features for downstream analysis. We applied this method in esophageal adenocarcinoma of different stages, identified the single-cell pathology landscape via reconstruction of WSI IMC images, and demonstrated the advantage of the dual-modality imaging strategy.","journal":"Cell Reports Methods","year":2023,"id":334093,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1063063,"name":"Phyllis Zixuan Chen","orcid":null,"position":1,"is_corresponding":false},{"id":94618,"name":"Dario Bressan","orcid":"0000-0003-3592-699X","position":2,"is_corresponding":false},{"id":239313,"name":"Monika Tripathi","orcid":null,"position":3,"is_corresponding":false},{"id":935623,"name":"Ahmad Miremadi","orcid":null,"position":4,"is_corresponding":false},{"id":238293,"name":"Massimiliano di Pietro","orcid":"0000-0003-4866-7026","position":5,"is_corresponding":false},{"id":90464,"name":"Lisa M. Coussens","orcid":"0000-0003-2389-1865","position":6,"is_corresponding":false},{"id":11809,"name":"Gregory J. Hannon","orcid":"0000-0003-4021-3898","position":7,"is_corresponding":false},{"id":13457,"name":"Rebecca C. Fitzgerald","orcid":"0000-0002-3434-3568","position":8,"is_corresponding":false},{"id":935134,"name":"Lizhe Zhuang","orcid":"0000-0002-7924-8919","position":9,"is_corresponding":false},{"id":225135,"name":"Young Hwan Chang","orcid":"0000-0001-8764-1959","position":10,"is_corresponding":false},{"id":935132,"name":"Eun Na Kim","orcid":"0000-0003-2992-7881","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-19T01:09:48.843291Z","pmid":"37741277","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}