{"doi":"10.1016/j.crmeth.2022.100199","title":"Multimodal imaging of synaptic vesicles with a single probe","abstract":"A complete understanding of synaptic-vesicle recycling requires the use of multiple microscopy methods to obtain complementary information. However, many currently available probes are limited to a specific microscopy modality, which necessitates the use of multiple probes and labeling paradigms. Given the complexity of vesicle populations and recycling pathways, having new single-vesicle probes that could be used for multiple microscopy techniques would complement existing sets of tools for studying vesicle function. Here, we present a probe based on the membrane-binding C2 domain of cytosolic phospholipase A2 (cPLA2) that fulfills this need. By conjugating the C2 domain with different detectable tags, we demonstrate that a single, modular probe can allow synaptic vesicles to be imaged at multiple levels of spatial and temporal resolution. Moreover, as a general endocytic marker, the C2 domain may also be used to study membrane recycling in many cell types.","journal":"Cell Reports Methods","year":2022,"id":289242,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9535,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":970866,"name":"Massimiliano Stagi","orcid":null,"position":1,"is_corresponding":false},{"id":426442,"name":"Travis J. Gould","orcid":"0009-0007-9416-1890","position":2,"is_corresponding":false},{"id":56619,"name":"Yumei Wu","orcid":"0000-0003-0878-7605","position":3,"is_corresponding":false},{"id":970427,"name":"Michael J. Mlodzianoski","orcid":"0000-0002-3510-9167","position":4,"is_corresponding":false},{"id":299630,"name":"Félix Rivera-Molina","orcid":"0000-0003-3772-497X","position":5,"is_corresponding":false},{"id":299631,"name":"Derek Toomre","orcid":"0000-0002-6827-8127","position":6,"is_corresponding":false},{"id":326241,"name":"Stephen M. Strittmatter","orcid":"0000-0001-8188-3092","position":7,"is_corresponding":false},{"id":56624,"name":"Pietro De Camilli","orcid":"0000-0001-9045-0723","position":8,"is_corresponding":false},{"id":244374,"name":"Joerg Bewersdorf","orcid":"0000-0002-4085-7020","position":9,"is_corresponding":false},{"id":492083,"name":"David Zenisek","orcid":"0000-0001-6052-0348","position":10,"is_corresponding":false},{"id":686759,"name":"Seong Jin An","orcid":"0000-0001-7981-4418","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-19T00:30:18.766838Z","pmid":"35497490","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}