{"doi":"10.1016/j.crmeth.2021.100117","title":"Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells","abstract":"High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling induced by SARS-CoV-2 at the whole-cell level with high spatial resolution and throughput. Most of the current SXT systems suffer from a restricted field of view due to use of flat sample supports and artifacts due to missing data. In this approach using cylindrical sample holders, a full-rotation tomogram of human lung epithelial cells is performed in less than 10 min. We demonstrate the potential of SXT imaging by visualizing aggregates of SARS-CoV-2 virions and virus-induced intracellular alterations. This rapid whole-cell imaging approach allows us to visualize the spatiotemporal changes of cellular organelles upon viral infection in a quantitative manner.","journal":"Cell Reports Methods","year":2021,"id":156356,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9439,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":321656,"name":"Jian-Hua Chen","orcid":"0000-0002-7998-0878","position":1,"is_corresponding":false},{"id":557217,"name":"Mirko Cortese","orcid":"0000-0003-1786-4211","position":2,"is_corresponding":false},{"id":321657,"name":"Axel Ekman","orcid":"0000-0002-4210-2617","position":3,"is_corresponding":false},{"id":480186,"name":"Mark A. Le Gros","orcid":null,"position":4,"is_corresponding":false},{"id":79422,"name":"Carolyn A. Larabell","orcid":"0000-0002-6262-4789","position":5,"is_corresponding":false},{"id":68297,"name":"Ralf Bartenschlager","orcid":"0000-0001-5601-9307","position":6,"is_corresponding":false},{"id":495969,"name":"Venera Weinhardt","orcid":"0000-0002-9774-3833","position":7,"is_corresponding":false},{"id":660640,"name":"Valentina Loconte","orcid":"0000-0001-5715-9993","position":0,"is_corresponding":true}],"reference_count":119,"raw_metadata":null,"created_at":"2026-07-18T23:44:08.368845Z","pmid":"34729550","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}