{"doi":"10.1016/j.clim.2020.108360","title":"B cells in rheumatoid arthritis synovial tissues encode focused antibody repertoires that include antibodies that stimulate macrophage TNF-α production","abstract":"Rheumatoid arthritis (RA) is characterized by the production of anti-citrullinated protein antibodies (ACPAs). To gain insights into the relationship between ACPA-expressing B cells in peripheral blood (PB) and synovial tissue (ST), we sequenced the B cell repertoire in paired PB and ST samples from five individuals with established, ACPA+ RA. Bioinformatics analysis of paired heavy- and light-chain sequences revealed clonally-related family members shared between PB and ST. ST-derived antibody repertoires exhibited reduced diversity and increased normalized clonal family size compared to PB-derived repertoires. Functional characterization showed that seven recombinant antibodies (rAbs) expressed from subject-derived sequences from both compartments bound citrullinated antigens and immune complexes (ICs) formed using one ST-derived rAb stimulated macrophage TNF-α production. Our findings demonstrate B cell trafficking between PB and ST in subjects with RA and ST repertoires include B cells that encode ACPA capable of forming ICs that stimulate cellular responses implicated in RA pathogenesis.","journal":"Clinical Immunology","year":2020,"id":62706,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9353,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":331612,"name":"Sarah Kongpachith","orcid":"0000-0002-8462-6881","position":1,"is_corresponding":false},{"id":332988,"name":"Nithya Lingampalli","orcid":null,"position":2,"is_corresponding":false},{"id":331613,"name":"Julia Z. Adamska","orcid":"0000-0001-8657-4141","position":3,"is_corresponding":false},{"id":331614,"name":"Bryan J. Cannon","orcid":"0000-0003-4418-1803","position":4,"is_corresponding":false},{"id":331615,"name":"Lisa K. Blum","orcid":"0000-0002-7921-8939","position":5,"is_corresponding":false},{"id":331616,"name":"Michelle S. Bloom","orcid":"0000-0002-5755-3955","position":6,"is_corresponding":false},{"id":332989,"name":"Matthew Henkel","orcid":null,"position":7,"is_corresponding":false},{"id":226238,"name":"Mandy J. McGeachy","orcid":"0000-0001-5029-1668","position":8,"is_corresponding":false},{"id":27563,"name":"Larry W. Moreland","orcid":"0000-0003-4483-0359","position":9,"is_corresponding":false},{"id":226251,"name":"William H. Robinson","orcid":"0000-0003-4385-704X","position":10,"is_corresponding":false},{"id":331611,"name":"Serra E. Elliott","orcid":"0000-0003-4808-7814","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-18T21:10:35.996198Z","pmid":"32035179","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}