{"doi":"10.1016/j.cj.2020.05.004","title":"Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization","abstract":"Phosphate deficiency is one of the leading causes of crop productivity loss. Phospholipid degradation liberates phosphate to cope with phosphate deficiency. Glycerophosphodiester phosphodiesterases (GPX-PDEs) hydrolyse the intermediate products of phospholipid catabolism glycerophosphodiesters into glycerol-3-phosphate, a precursor of phosphate. However, the function of GPX-PDEs in phosphate remobilization in maize remains unclear. In the present study, we characterized two phosphate deficiency-inducible GPX-PDE genes, ZmGPX-PDE1 and ZmGPX-PDE5, in maize leaves. ZmGPX-PDE1 and ZmGPX-PDE5 were transcriptionally regulated by ZmPHR1, a well-described phosphate starvation-responsive transcription factor of the MYB family. Complementation of the yeast GPX-PDE mutant gde1∆ indicated that ZmGPX-PDE1 and ZmGPX-PDE5 functioned as GPX-PDEs, suggesting their roles in phosphate recycling from glycerophosphodiesters. In vitro enzyme assays showed that ZmGPX-PDE1 and ZmGPX-PDE5 catalysed glycerophosphodiester degradation with different substrate preferences for glycerophosphoinositol and glycerophosphocholine, respectively. ZmGPX-PDE1 was upregulated during leaf senescence, and more remarkably, loss of ZmGPX-PDE1 in maize compromised the remobilization of phosphorus from senescing leaves to young leaves, resulting in a stay-green phenotype under phosphate starvation. These results suggest that ZmGPX-PDE1 catalyses the degradation of glycerophosphodiesters in maize, promoting phosphate recycling from senescing leaves to new leaves. This mechanism is crucial for improving phosphorus utilization efficiency in crops.","journal":"The Crop Journal","year":2020,"id":93327,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":40,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9528,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":465963,"name":"Wenbo Pan","orcid":"0000-0001-9734-3090","position":1,"is_corresponding":false},{"id":466755,"name":"Alexiy Nikiforov","orcid":null,"position":2,"is_corresponding":false},{"id":465964,"name":"William R. King","orcid":"0000-0001-5292-975X","position":3,"is_corresponding":false},{"id":466756,"name":"Wanting Hong","orcid":null,"position":4,"is_corresponding":false},{"id":465965,"name":"Weiwei Li","orcid":"0000-0001-7921-3727","position":5,"is_corresponding":false},{"id":465966,"name":"Yang Han","orcid":"0000-0001-5528-8708","position":6,"is_corresponding":false},{"id":465967,"name":"Jana Patton‐Vogt","orcid":"0000-0003-2189-3664","position":7,"is_corresponding":false},{"id":465968,"name":"Jianbo Shen","orcid":"0000-0001-8943-948X","position":8,"is_corresponding":false},{"id":465969,"name":"Lingyun Cheng","orcid":"0000-0001-5185-1196","position":9,"is_corresponding":false},{"id":321302,"name":"Jingxin Wang","orcid":"0000-0002-9414-4093","position":0,"is_corresponding":true}],"reference_count":60,"raw_metadata":null,"created_at":"2026-07-18T22:30:54.208860Z","pmid":"40735295","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}