{"doi":"10.1016/j.chom.2023.10.018","title":"Broad receptor tropism and immunogenicity of a clade 3 sarbecovirus","abstract":"Although Rhinolophus bats harbor diverse clade 3 sarbecoviruses, the structural determinants of receptor tropism along with the antigenicity of their spike (S) glycoproteins remain uncharacterized. Here, we show that the African Rhinolophus bat clade 3 sarbecovirus PRD-0038 S has a broad angiotensin-converting enzyme 2 (ACE2) usage and that receptor-binding domain (RBD) mutations further expand receptor promiscuity and enable human ACE2 utilization. We determine a cryo-EM structure of the PRD-0038 RBD bound to Rhinolophus alcyone ACE2, explaining receptor tropism and highlighting differences with SARS-CoV-1 and SARS-CoV-2. Characterization of PRD-0038 S using cryo-EM and monoclonal antibody reactivity reveals its distinct antigenicity relative to SARS-CoV-2 and identifies PRD-0038 cross-neutralizing antibodies for pandemic preparedness. PRD-0038 S vaccination elicits greater titers of antibodies cross-reacting with vaccine-mismatched clade 2 and clade 1a sarbecoviruses compared with SARS-CoV-2 S due to broader antigenic targeting, motivating the inclusion of clade 3 antigens in next-generation vaccines for enhanced resilience to viral evolution.","journal":"Cell Host & Microbe","year":2023,"id":321776,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":614554,"name":"Samantha K. Zepeda","orcid":"0000-0003-0005-9793","position":1,"is_corresponding":false},{"id":97052,"name":"Young‐Jun Park","orcid":"0000-0003-2901-6949","position":2,"is_corresponding":false},{"id":1028033,"name":"Ashley L. Taylor","orcid":"0000-0001-9975-7596","position":3,"is_corresponding":false},{"id":623126,"name":"Joel Quispe","orcid":"0000-0002-3693-4935","position":4,"is_corresponding":false},{"id":828534,"name":"Cameron Stewart","orcid":"0000-0002-2786-6256","position":5,"is_corresponding":false},{"id":672274,"name":"Elizabeth M. Leaf","orcid":"0000-0002-5354-6010","position":6,"is_corresponding":false},{"id":1034563,"name":"Catherine Treichel","orcid":"0000-0002-6024-8505","position":7,"is_corresponding":false},{"id":80082,"name":"Davide Corti","orcid":"0000-0002-5797-1364","position":8,"is_corresponding":false},{"id":108915,"name":"Neil P. King","orcid":"0000-0002-2978-4692","position":9,"is_corresponding":false},{"id":106706,"name":"Tyler N. Starr","orcid":"0000-0001-6713-6904","position":10,"is_corresponding":false},{"id":97051,"name":"David Veesler","orcid":"0000-0002-6019-8675","position":11,"is_corresponding":false},{"id":1034562,"name":"Jimin Lee","orcid":"0000-0001-5849-7732","position":0,"is_corresponding":true}],"reference_count":123,"raw_metadata":null,"created_at":"2026-07-19T01:07:37.596550Z","pmid":"37989312","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}