{"doi":"10.1016/j.chom.2023.08.006","title":"Spontaneous HIV expression during suppressive ART is associated with the magnitude and function of HIV-specific CD4+ and CD8+ T cells","abstract":"Spontaneous transcription and translation of HIV can persist during suppressive antiretroviral therapy (ART). The quantity, phenotype, and biological relevance of this spontaneously \"active\" reservoir remain unclear. Using multiplexed single-cell RNAflow-fluorescence in situ hybridization (FISH), we detect active HIV transcription in 14/18 people with HIV on suppressive ART, with a median of 28/million CD4 + T cells. While these cells predominantly exhibit abortive transcription, p24-expressing cells are evident in 39% of participants. Phenotypically diverse, active reservoirs are enriched in central memory T cells and CCR6- and activation-marker-expressing cells. The magnitude of the active reservoir positively correlates with total HIV-specific CD4 + and CD8 + T cell responses and with multiple HIV-specific T cell clusters identified by unsupervised analysis. These associations are particularly strong with p24-expressing active reservoir cells. Single-cell vDNA sequencing shows that active reservoirs are largely dominated by defective proviruses. Our data suggest that these reservoirs maintain HIV-specific CD4 + and CD8 + T responses during suppressive ART.","journal":"Cell Host & Microbe","year":2023,"id":317183,"datarank":0.6646225198264971,"base_score":4.430816798843313,"endowment":4.430816798843313,"self_citation_contribution":0.6646225198264971,"citation_network_contribution":0.0,"self_endowment_contribution":0.6646225198264971,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":83,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9637,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":623261,"name":"Olivier Tastet","orcid":"0000-0001-5694-6266","position":1,"is_corresponding":false},{"id":656425,"name":"Caroline Dufour","orcid":"0000-0002-2560-5853","position":2,"is_corresponding":false},{"id":347020,"name":"Gérémy Sannier","orcid":"0000-0001-7956-4463","position":3,"is_corresponding":false},{"id":347021,"name":"Nathalie Brassard","orcid":"0000-0002-4320-9416","position":4,"is_corresponding":false},{"id":349169,"name":"Gloria-Gabrielle Delgado","orcid":null,"position":5,"is_corresponding":false},{"id":399936,"name":"Amélie Pagliuzza","orcid":"0000-0001-8124-2607","position":6,"is_corresponding":false},{"id":91572,"name":"Corentin Richard","orcid":"0000-0001-9732-0219","position":7,"is_corresponding":false},{"id":623260,"name":"Manon Nayrac","orcid":"0000-0001-5717-6232","position":8,"is_corresponding":false},{"id":233493,"name":"Jean‐Pierre Routy","orcid":"0000-0001-9897-7589","position":9,"is_corresponding":false},{"id":322674,"name":"Alexandre Prat","orcid":"0000-0001-6188-0580","position":10,"is_corresponding":false},{"id":95245,"name":"Jacob D. Estes","orcid":"0000-0002-9652-6394","position":11,"is_corresponding":false},{"id":342350,"name":"Rémi Fromentin","orcid":"0000-0002-8153-1799","position":12,"is_corresponding":false},{"id":235294,"name":"Nicolas Chomont","orcid":"0000-0001-9747-5018","position":13,"is_corresponding":false},{"id":254288,"name":"Daniel E. Kaufmann","orcid":"0000-0003-4467-136X","position":14,"is_corresponding":false},{"id":254285,"name":"Mathieu Dubé","orcid":"0000-0002-5522-9019","position":0,"is_corresponding":true}],"reference_count":92,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:06:50.336418Z","pmid":"37708853","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}