{"doi":"10.1016/j.chom.2022.03.034","title":"A single-cell liver atlas of Plasmodium vivax infection","abstract":"Malaria-causing Plasmodium vivax parasites can linger in the human liver for weeks to years and reactivate to cause recurrent blood-stage infection. Although they are an important target for malaria eradication, little is known about the molecular features of replicative and non-replicative intracellular liver-stage parasites and their host cell dependence. Here, we leverage a bioengineered human microliver platform to culture patient-derived P. vivax parasites for transcriptional profiling. Coupling enrichment strategies with bulk and single-cell analyses, we capture both parasite and host transcripts in individual hepatocytes throughout the course of infection. We define host- and state-dependent transcriptional signatures and identify unappreciated populations of replicative and non-replicative parasites that share features with sexual transmissive forms. We find that infection suppresses the transcription of key hepatocyte function genes and elicits an anti-parasite innate immune response. Our work provides a foundation for understanding host-parasite interactions and reveals insights into the biology of P. vivax dormancy and transmission.","journal":"Cell Host & Microbe","year":2022,"id":234427,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9315,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":850474,"name":"Nil Gural","orcid":null,"position":1,"is_corresponding":false},{"id":849983,"name":"Eliana Real","orcid":"0000-0002-2502-9374","position":2,"is_corresponding":false},{"id":19166,"name":"Marc H. Wadsworth","orcid":"0000-0001-9479-8516","position":3,"is_corresponding":false},{"id":48125,"name":"Vincent L. Butty","orcid":"0000-0003-1173-2429","position":4,"is_corresponding":false},{"id":634088,"name":"Sandra March","orcid":"0000-0003-1617-7110","position":5,"is_corresponding":false},{"id":849984,"name":"Niketa Nerurkar","orcid":"0000-0002-7707-9373","position":6,"is_corresponding":false},{"id":19167,"name":"Travis K. Hughes","orcid":null,"position":7,"is_corresponding":false},{"id":334656,"name":"Wanlapa Roobsoong","orcid":"0000-0002-1789-0439","position":8,"is_corresponding":false},{"id":281732,"name":"Heather E. Fleming","orcid":"0000-0002-5286-3794","position":9,"is_corresponding":false},{"id":54226,"name":"Charles A. Whittaker","orcid":"0000-0002-2400-0094","position":10,"is_corresponding":false},{"id":62836,"name":"Stuart S. Levine","orcid":"0000-0001-7363-562X","position":11,"is_corresponding":false},{"id":334155,"name":"Jetsumon Sattabongkot","orcid":"0000-0002-3938-4588","position":12,"is_corresponding":false},{"id":1413,"name":"Alex K. Shalek","orcid":"0000-0001-5670-8778","position":13,"is_corresponding":false},{"id":7941,"name":"Sangeeta N. Bhatia","orcid":"0000-0002-1293-2097","position":14,"is_corresponding":false},{"id":849982,"name":"Liliana Mâncio-Silva","orcid":"0000-0002-5585-018X","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T00:21:36.529874Z","pmid":"35443155","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}