{"doi":"10.1016/j.chembiol.2023.12.017","title":"Bioorthogonal click labeling of an amber-free HIV-1 provirus for in-virus single molecule imaging","abstract":"Structural dynamics of human immunodeficiency virus 1 (HIV-1) envelope (Env) glycoprotein mediate cell entry and facilitate immune evasion. Single-molecule FRET using peptides for Env labeling revealed structural dynamics of Env, but peptide use risks potential effects on structural integrity/dynamics. While incorporating noncanonical amino acids (ncAAs) into Env by amber stop-codon suppression, followed by click chemistry, offers a minimally invasive approach, this has proved to be technically challenging for HIV-1. Here, we develope an intact amber-free HIV-1 system that overcomes hurdles of preexisting viral amber codons. We achieved dual-ncAA incorporation into Env on amber-free virions, enabling single-molecule Förster resonance energy transfer (smFRET) studies of click-labeled Env that validated the previous peptide-based labeling approaches by confirming the intrinsic propensity of Env to dynamically sample multiple conformational states. Amber-free click-labeled Env also enabled real-time tracking of single virion internalization and trafficking in cells. Our system thus permits in-virus bioorthogonal labeling of proteins, compatible with studies of virus entry, trafficking, and egress from cells.","journal":"Cell chemical biology","year":2024,"id":426308,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":241929,"name":"Jonathan R. Grover","orcid":"0000-0003-2137-600X","position":1,"is_corresponding":false},{"id":1172737,"name":"Levi B. Gifford","orcid":"0000-0002-6324-6919","position":2,"is_corresponding":false},{"id":1171366,"name":"Yang Han","orcid":"0000-0002-0034-5848","position":3,"is_corresponding":false},{"id":996471,"name":"Guohua Zhong","orcid":"0000-0003-0207-4890","position":4,"is_corresponding":false},{"id":1225217,"name":"Revansiddha Katte","orcid":"0000-0002-3844-8797","position":5,"is_corresponding":false},{"id":241925,"name":"Wenwei Li","orcid":"0000-0003-3008-6901","position":6,"is_corresponding":false},{"id":1171667,"name":"Rajanya Bhattacharjee","orcid":null,"position":7,"is_corresponding":false},{"id":229158,"name":"Baoshan Zhang","orcid":"0000-0002-0165-1513","position":8,"is_corresponding":false},{"id":1044636,"name":"Stephanie Sauve","orcid":null,"position":9,"is_corresponding":false},{"id":742123,"name":"Wenyi Qin","orcid":"0000-0001-5460-0325","position":10,"is_corresponding":false},{"id":798429,"name":"Dibya Ghimire","orcid":null,"position":11,"is_corresponding":false},{"id":1019937,"name":"Md. Anzarul Haque","orcid":"0000-0002-4603-6565","position":12,"is_corresponding":false},{"id":367028,"name":"James Arthos","orcid":"0000-0002-6767-4267","position":13,"is_corresponding":false},{"id":564984,"name":"Mahmoud Moradi","orcid":"0000-0002-0601-402X","position":14,"is_corresponding":false},{"id":241931,"name":"Walther Mothes","orcid":"0000-0002-3367-7240","position":15,"is_corresponding":false},{"id":257874,"name":"Edward A. Lemke","orcid":"0000-0002-0634-0503","position":16,"is_corresponding":false},{"id":105633,"name":"Peter D. Kwong","orcid":"0000-0003-3560-232X","position":17,"is_corresponding":false},{"id":141191,"name":"Gregory B. Melikyan","orcid":"0000-0001-5385-3013","position":18,"is_corresponding":false},{"id":241923,"name":"Maolin Lu","orcid":"0000-0002-8099-6447","position":19,"is_corresponding":false},{"id":1171365,"name":"Yuanyun Ao","orcid":"0000-0002-0845-8994","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-19T01:58:37.924718Z","pmid":"38232732","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}