{"doi":"10.1016/j.chembiol.2008.11.004","title":"Novel Fluorescent Glycan Microarray Strategy Reveals Ligands for Galectins","abstract":null,"journal":"Chemistry & Biology","year":2009,"id":592013,"datarank":6.476482248597398,"base_score":5.472270673671475,"endowment":5.472270673671475,"self_citation_contribution":0.8208406010507213,"citation_network_contribution":5.655641647546676,"self_endowment_contribution":0.8208406010507213,"citer_contribution":5.655641647546676,"corpus_percentile":null,"corpus_rank":null,"citation_count":237,"citer_count":155,"citers_with_citation_signal":143,"citers_with_endowment":143,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":346013,"name":"Baoyun Xia","orcid":"0000-0001-5081-7447","position":1,"is_corresponding":false},{"id":226169,"name":"Sean R. Stowell","orcid":"0000-0002-1130-9551","position":2,"is_corresponding":false},{"id":434512,"name":"Yi Lasanajak","orcid":"0000-0003-1450-8107","position":3,"is_corresponding":false},{"id":163459,"name":"David F. Smith","orcid":null,"position":4,"is_corresponding":false},{"id":231529,"name":"Richard D. Cummings","orcid":"0000-0002-8918-5034","position":5,"is_corresponding":false},{"id":373260,"name":"Xuezheng Song","orcid":"0000-0003-1751-2785","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Novel Fluorescent Glycan Microarray Strategy Reveals Ligands for Galectins","abstract":"Galectin-1 (Gal-1) and galectin-3 (Gal-3) are widely expressed galectins with immunoregulatory functions in animals. To explore their glycan specificity, we developed microarrays of naturally occurring glycans using a bifunctional fluorescent linker, 2-amino-N-(2-aminoethyl)-benzamide (AEAB), directly conjugated through its arylamine group by reductive amination to free glycans to form glycan-AEABs (GAEABs). Glycans from natural sources were used to prepare over 200 GAEABs, which were purified by multidimensional high-pressure liquid chromatography and covalently immobilized onto N-hydroxysuccinimide-activated glass slides via their free alkylamine. Fluorescence-based screening demonstrated that Gal-1 recognizes a wide variety of complex N-glycans, whereas Gal-3 primarily recognizes poly-N-acetyllactosamine-containing glycans independent of N-glycan presentation. GAEABs provide a general solution to glycan microarray preparation from natural sources for defining the specificity of glycan-binding proteins.","is_dataset_classified":null,"base_score":5.472270673671475,"endowment":5.472270673671475,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19171304","pmcid":"PMC2662446","openalex_id":"https://openalex.org/W2083556833","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM085448","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM085448","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM62116","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008169","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"U54 GM062116","title":null},{"funder_name":"National Institutes of Health","grant_id":"1U54GM062116-01A1","title":"PILOT--ANTIGENS AND T CELL RECOGNITION OF CARBOHYDRATES"}],"total_grants":6,"fwci":7.3502,"citation_percentile":0.97791447,"influential_citations":0,"citation_trend":[{"year":2012,"count":13},{"year":2013,"count":13},{"year":2014,"count":20},{"year":2015,"count":11},{"year":2016,"count":15},{"year":2017,"count":15},{"year":2018,"count":8},{"year":2019,"count":21},{"year":2020,"count":13},{"year":2021,"count":10},{"year":2022,"count":13},{"year":2023,"count":14},{"year":2024,"count":9},{"year":2025,"count":10}],"oa_status":"green","license":"Elsevier Non-Commercial","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2662446","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2662446","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S107455210800450X?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S107455210800450X?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.chembiol.2008.11.004","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19171304","host_type":"repository"},{"url":"http://dx.doi.org/10.1016/j.chembiol.2008.11.004","host_type":""},{"url":"https://dx.doi.org/10.1016/j.chembiol.2008.11.004","host_type":""}],"fields_of_study":["Galectins and Cancer Biology","Glycosylation and Glycoproteins Research","Signaling Pathways in Disease","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Benzamides","Chromatography, High Pressure Liquid","Fluorescent Dyes","Galectin 1","Galectin 3","Galectins","Ligands","Microarray Analysis","Polysaccharides","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization"],"mesh_terms":["Animals","Benzamides","Chromatography, High Pressure Liquid","Fluorescent Dyes","Ligands","Polysaccharides","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Galectins","Galectin 1","Galectin 3","Microarray Analysis"],"keywords":["Glycan","Galectin","Linker","Reductive amination","Fluorescence","Biochemistry","Bifunctional","Chemistry","Biology","Combinatorial chemistry","Glycoprotein","Pharmacology","Galectin 1","Galectin 3","Galectins","Clinical Biochemistry","Ligands","Microarray Analysis","CHEMBIO","Polysaccharides","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Drug Discovery","Benzamides","Molecular Medicine","Animals","MOLIMMUNO","Molecular Biology","Chromatography, High Pressure Liquid","Fluorescent Dyes"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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