{"doi":"10.1016/j.cels.2023.04.002","title":"A vast evolutionarily transient translatome contributes to phenotype and fitness","abstract":"Translation is the process by which ribosomes synthesize proteins. Ribosome profiling recently revealed that many short sequences previously thought to be noncoding are pervasively translated. To identify protein-coding genes in this noncanonical translatome, we combine an integrative framework for extremely sensitive ribosome profiling analysis, iRibo, with high-powered selection inferences tailored for short sequences. We construct a reference translatome for Saccharomyces cerevisiae comprising 5,400 canonical and almost 19,000 noncanonical translated elements. Only 14 noncanonical elements were evolving under detectable purifying selection. A representative subset of translated elements lacking signatures of selection demonstrated involvement in processes including DNA repair, stress response, and post-transcriptional regulation. Our results suggest that most translated elements are not conserved protein-coding genes and contribute to genotype-phenotype relationships through fast-evolving molecular mechanisms.","journal":"Cell Systems","year":2023,"id":318402,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":65,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":256290,"name":"Saurin Bipin Parikh","orcid":"0000-0002-8167-5768","position":1,"is_corresponding":false},{"id":256286,"name":"Nelson Castilho Coelho","orcid":"0000-0002-8331-5889","position":2,"is_corresponding":false},{"id":256284,"name":"Omer Acar","orcid":"0000-0001-5361-256X","position":3,"is_corresponding":false},{"id":808915,"name":"Carly Houghton","orcid":"0000-0003-0158-3044","position":4,"is_corresponding":false},{"id":808916,"name":"Lin Chou","orcid":"0000-0002-0227-5521","position":5,"is_corresponding":false},{"id":17800,"name":"Anne-Ruxandra Carvunis","orcid":"0000-0002-6474-6413","position":6,"is_corresponding":false},{"id":78844,"name":"Aaron Wacholder","orcid":"0000-0001-8739-0029","position":0,"is_corresponding":true}],"reference_count":95,"raw_metadata":null,"created_at":"2026-07-19T01:07:01.300821Z","pmid":"37164009","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}