{"doi":"10.1016/j.celrep.2025.116677","title":"Coordinated changes in stromal and hematopoietic cells that define the perinatal to juvenile transition in the mouse thymus","abstract":"Perinatal T cells have distinctive phenotypes and functions that may be due in part to age-associated features of stromal cells in the perinatal thymus. We identify age-associated changes in mouse thymic epithelial cells, mesenchyme, endothelium, and hematopoietic antigen-presenting cells from birth to one month of age using single-cell transcriptional profiling, flow cytometry, and imaging. Coordinated cellular and molecular changes occur at 7-14 days of age, designated \"transitional ages,\" as thymus growth switches to homeostasis. E2F target gene expression declines, and the expression of type I interferon response genes increases across diverse cell types at transitional ages. Alterations in thymic stromal cells coincide with elevated markers of thymocyte self-reactivity and enhanced Treg suppressive phenotypes and function. The integrated results reveal coordinated remodeling of multiple stromal cell types during the perinatal to juvenile transition, which likely impacts T cell differentiation. These datasets provide a resource for the investigation of the perinatal thymus environment.","journal":"Cell Reports","year":2025,"id":535742,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9477,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1419904,"name":"Colin E. Moore","orcid":"0000-0001-7277-7343","position":1,"is_corresponding":false},{"id":1420405,"name":"Aparna Calindi","orcid":null,"position":2,"is_corresponding":false},{"id":307613,"name":"Seungwoo Kang","orcid":"0000-0003-1301-8480","position":3,"is_corresponding":false},{"id":367398,"name":"Bryan R. Helm","orcid":"0000-0003-3386-7842","position":4,"is_corresponding":false},{"id":236809,"name":"Jayashree Srinivasan","orcid":"0000-0001-9478-7518","position":5,"is_corresponding":false},{"id":1420406,"name":"Siddhartha Shah","orcid":null,"position":6,"is_corresponding":false},{"id":1419905,"name":"Erin Baker","orcid":"0000-0002-4139-9930","position":7,"is_corresponding":false},{"id":1420407,"name":"Ruiting Zong","orcid":null,"position":8,"is_corresponding":false},{"id":1420408,"name":"Nandini Singarapu","orcid":null,"position":9,"is_corresponding":false},{"id":1420409,"name":"Scott Casey","orcid":null,"position":10,"is_corresponding":false},{"id":225650,"name":"Andrew N. Macintyre","orcid":"0000-0002-5297-0207","position":11,"is_corresponding":false},{"id":60556,"name":"Ken S. Lau","orcid":"0000-0001-8438-0319","position":12,"is_corresponding":false},{"id":432477,"name":"Laura P. Hale","orcid":"0000-0001-5707-0390","position":13,"is_corresponding":false},{"id":692556,"name":"Qi Liu","orcid":"0000-0001-8892-7078","position":14,"is_corresponding":false},{"id":488525,"name":"Nancy R. Manley","orcid":"0000-0001-8489-7764","position":15,"is_corresponding":false},{"id":236814,"name":"Lauren I. R. Ehrlich","orcid":"0000-0002-1697-1755","position":16,"is_corresponding":false},{"id":987557,"name":"Ellen R. Richie","orcid":"0000-0003-0786-973X","position":17,"is_corresponding":false},{"id":357061,"name":"Anusha Vasudev","orcid":"0000-0001-8901-2451","position":0,"is_corresponding":true}],"reference_count":120,"raw_metadata":null,"created_at":"2026-07-19T02:52:00.885532Z","pmid":"41389204","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}