{"doi":"10.1016/j.celrep.2025.116280","title":"Neurons and astrocytes have distinct organelle signatures and responses to stress","abstract":"Neurons and astrocytes play critical yet divergent roles in brain physiology and neurological conditions. Intracellular organelles are integral to cellular function. However, an in-depth characterization of organelles in live neural cells has not been performed. Here, we use multispectral imaging to simultaneously visualize six organelles-endoplasmic reticulum (ER), lysosomes, mitochondria, peroxisomes, Golgi, and lipid droplets-in live primary rodent neurons and astrocytes. We generate a dataset of 173 z stack and 98 time-lapse images, accompanied by quantitative \"organelle signature\" analysis. Comparative analysis reveals a clear cell-type specificity in organelle morphology and interactions. Neurons are characterized by prominent mitochondrial composition and interactions, while astrocytes contain more lysosomes and lipid droplet interactions. Additionally, neurons display a more robust organelle response than astrocytes to acute oxidative or ER stress. Our data provide a systems-level characterization of neuron and astrocyte organelles that can be a reference for understanding cell-type-specific physiology and disease.","journal":"Cell Reports","year":2025,"id":514505,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1377732,"name":"Weizhen Dong","orcid":null,"position":1,"is_corresponding":false},{"id":1231349,"name":"Chih-Hsuan Hsu","orcid":"0000-0002-5599-807X","position":2,"is_corresponding":false},{"id":1377733,"name":"Ngudiankama R. Mfulama","orcid":null,"position":3,"is_corresponding":false},{"id":1377734,"name":"Ridhi Yarlagadda","orcid":null,"position":4,"is_corresponding":false},{"id":821753,"name":"Joey V. Ragusa","orcid":"0009-0008-4779-9370","position":5,"is_corresponding":false},{"id":1377144,"name":"Michael Ye","orcid":"0000-0002-6884-6067","position":6,"is_corresponding":false},{"id":1377735,"name":"Andy Henrie","orcid":null,"position":7,"is_corresponding":false},{"id":509716,"name":"Maria Clara Zanellati","orcid":"0000-0003-0597-4923","position":8,"is_corresponding":false},{"id":714544,"name":"Graham H. Diering","orcid":"0000-0001-6113-6946","position":9,"is_corresponding":false},{"id":280536,"name":"Todd J. Cohen","orcid":"0000-0002-4099-0278","position":10,"is_corresponding":false},{"id":333835,"name":"Sarah Cohen","orcid":"0000-0003-2982-3265","position":11,"is_corresponding":false},{"id":511313,"name":"Shannon N. Rhoads","orcid":"0000-0003-2391-0392","position":0,"is_corresponding":true}],"reference_count":134,"raw_metadata":null,"created_at":"2026-07-19T02:48:29.165853Z","pmid":"40956669","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}