{"doi":"10.1016/j.celrep.2025.116133","title":"Promoter- and enhancer-dependent cohesin loading initiates chromosome looping to fold Tcrb loci for long-range recombination","abstract":"Cohesin-mediated chromosome looping regulates diverse processes, including antigen receptor (AgR) gene assembly by V(D)J recombination. To understand the mechanisms that coordinate genome topologies, we focused on a genetically tractable AgR locus, Tcrb. Cohesin loading and initiation of loop extrusion (LE) from a nearby CTCF-binding element (CBE) required the promoter of the most 5'Vb segment, creating long-range contacts with downstream DJb segments in the recombination center (RC). CBEs flanking the RC have multiple functions: termination of LE originating in the Vb cluster, initiation of LE in the RC, and insulation of enhancer activity. Deletion of the Tcrb super-enhancer abolished LE from the neighboring RC but spared long-range contacts, indicating that unidirectional LE from upstream Vb segments was sufficient. Thus, Vb promoter- or enhancer-dependent cohesin loading initiates LE in opposite directions across the locus to assemble a broad Tcrb repertoire, a finding that has broad implications for genomic architecture and function.","journal":"Cell Reports","year":2025,"id":546172,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9613,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":244454,"name":"Katharina E. Hayer","orcid":"0000-0002-1463-3111","position":1,"is_corresponding":false},{"id":289426,"name":"Clément Oyeniran","orcid":"0000-0002-4476-0891","position":2,"is_corresponding":false},{"id":244452,"name":"Vincent Nganga","orcid":"0000-0003-0879-6560","position":3,"is_corresponding":false},{"id":396901,"name":"Kyutae D. Lee","orcid":"0000-0002-0348-8019","position":4,"is_corresponding":false},{"id":691190,"name":"Ahmet Saçan","orcid":"0000-0002-3570-3270","position":5,"is_corresponding":false},{"id":244459,"name":"Eugene M. Oltz","orcid":"0000-0002-2513-7156","position":6,"is_corresponding":false},{"id":244458,"name":"Craig H. Bassing","orcid":"0000-0003-1703-0786","position":7,"is_corresponding":false},{"id":513207,"name":"Brittney M. Allyn","orcid":"0000-0003-1254-6097","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T02:53:32.285899Z","pmid":"40833849","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}