{"doi":"10.1016/j.celrep.2025.115351","title":"B cell stimulation changes the structure and higher-order organization of the inactive X chromosome","abstract":"X chromosome inactivation (XCI) equalizes X-linked gene expression between sexes. B cells exhibit dynamic XCI, with Xist RNA/heterochromatic marks absent on the inactive X (Xi) in naive B cells but returning following mitogenic stimulation. The impact of dynamic XCI on Xi structure and maintenance was previously unknown. Here, we find dosage compensation of the Xi with state-specific XCI escape genes in naive and in vitro-activated B cells. Allele-specific OligoPaints indicate similar Xi and active X (Xa) territories in B cells that are less compact than in fibroblasts. Allele-specific Hi-C reveals a lack of TAD-like structures on the Xi of naive B cells and stimulation-induced alterations in TAD-like boundary strength independent of gene expression. Notably, Xist deletion in B cells changes TAD boundaries and large-scale Xi compaction. Altogether, our results uncover B cell-specific Xi plasticity, which could underlie sex-biased biological mechanisms.","journal":"Cell Reports","year":2025,"id":519132,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9532,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1045648,"name":"Natalie E. Toothacre","orcid":"0000-0002-4500-7965","position":1,"is_corresponding":false},{"id":622909,"name":"Robin H. van der Weide","orcid":"0000-0002-6466-7280","position":2,"is_corresponding":false},{"id":237764,"name":"Claudia D. Lovell","orcid":"0000-0001-7944-5536","position":3,"is_corresponding":false},{"id":258048,"name":"Son C. Nguyen","orcid":"0000-0003-2187-1027","position":4,"is_corresponding":false},{"id":846397,"name":"R. Jordan Barnett","orcid":"0000-0002-7540-833X","position":5,"is_corresponding":false},{"id":829136,"name":"Ashley L. Cook","orcid":"0000-0001-6789-5732","position":6,"is_corresponding":false},{"id":415777,"name":"Han-Seul Ryu","orcid":"0000-0001-9938-4902","position":7,"is_corresponding":false},{"id":403001,"name":"Sarah Pyfrom","orcid":"0000-0002-3781-6463","position":8,"is_corresponding":false},{"id":1262250,"name":"Harrison Wang","orcid":"0000-0002-5311-1375","position":9,"is_corresponding":false},{"id":276893,"name":"Daniel P. Beiting","orcid":"0000-0002-2865-4589","position":10,"is_corresponding":false},{"id":401458,"name":"Jennifer E. Phillips‐Cremins","orcid":"0000-0002-4702-0450","position":11,"is_corresponding":false},{"id":326068,"name":"Eric F. Joyce","orcid":"0000-0002-0418-2804","position":12,"is_corresponding":false},{"id":376544,"name":"Montserrat C. Anguera","orcid":"0000-0003-4992-3079","position":13,"is_corresponding":false},{"id":991071,"name":"Isabel Sierra","orcid":"0000-0002-4875-2741","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-19T02:49:14.184995Z","pmid":"40014450","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}