{"doi":"10.1016/j.celrep.2024.115163","title":"Isolation and structure of broad SIV-neutralizing antibodies reveal a proximal helical MPER epitope recognized by a rhesus multi-donor class","abstract":"The membrane-proximal external region (MPER) of the HIV-1 envelope is a target for broadly neutralizing antibodies (bnAbs), and vaccine-elicited MPER-directed antibodies have recently been reported from a human clinical trial. In this study, we sought to identify MPER-directed nAbs in simian immunodeficiency virus (SIV)-infected rhesus macaques. We isolated four lineages of SIV MPER-directed nAbs from two SIV-infected macaques. The nAbs displayed low potency but up to 90% breadth on a 20-strain SIV panel. Crystal structures of representative nAbs in complex with SIV MPER peptides revealed the SIV antibodies to bind a helical epitope at the N-terminal (proximal) region of the MPER, defining a reproducible multi-donor class encompassing all four lineages. HIV-1 comparison showed that this class of SIV MPER-directed antibodies targets a helical region overlapping that targeted by human vaccine-elicited ones. Thus, a prevalent and reproducible class of SIV bnAbs recognizes an epitope similar to that recently observed in an HIV-1-vaccine trial.","journal":"Cell Reports","year":2025,"id":560096,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9591,"is_data_producer":true,"deposit_databanks":{"PDB":["9BP1","9BLX","9BNS"],"GENBANK":["OR756535","OR756536","OR756537","OR756538","OR756539","OR756540","OR756541","OR756542","OR756543","OR756544","OR756545","OR756546","OR756547","OR756548","OR756549","OR756550","OR756551","OR756552","OR756553","OR756554","OR756555","OR756556","OR756557","OR756558","OR756559","OR756560"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":910844,"name":"Renguang Du","orcid":null,"position":1,"is_corresponding":false},{"id":457173,"name":"Yen‐Ting Lai","orcid":"0000-0003-3700-6417","position":2,"is_corresponding":false},{"id":695998,"name":"Mohammed S. Ahmadi","orcid":null,"position":3,"is_corresponding":false},{"id":651894,"name":"Hannah A. D. King","orcid":"0000-0002-7575-198X","position":4,"is_corresponding":false},{"id":731906,"name":"Kaimei Song","orcid":null,"position":5,"is_corresponding":false},{"id":1462259,"name":"Kimberly Manalang","orcid":null,"position":6,"is_corresponding":false},{"id":715822,"name":"Christopher A. Gonelli","orcid":"0000-0002-7521-8528","position":7,"is_corresponding":false},{"id":283220,"name":"Chaim A. Schramm","orcid":null,"position":8,"is_corresponding":false},{"id":1267664,"name":"Cheng Cheng","orcid":"0000-0002-3837-9293","position":9,"is_corresponding":false},{"id":280563,"name":"Richard Nguyen","orcid":"0000-0003-2453-3461","position":10,"is_corresponding":false},{"id":280561,"name":"David R. Ambrozak","orcid":"0000-0003-1038-7328","position":11,"is_corresponding":false},{"id":324811,"name":"Aliaksandr Druz","orcid":null,"position":12,"is_corresponding":false},{"id":390894,"name":"Chen‐Hsiang Shen","orcid":"0000-0002-9560-3992","position":13,"is_corresponding":false},{"id":457175,"name":"Yongping Yang","orcid":"0000-0002-8307-1095","position":14,"is_corresponding":false},{"id":277153,"name":"Daniel C. Douek","orcid":"0000-0001-5575-8634","position":15,"is_corresponding":false},{"id":105633,"name":"Peter D. Kwong","orcid":"0000-0003-3560-232X","position":16,"is_corresponding":false},{"id":18050,"name":"Mario Roederer","orcid":"0000-0001-7448-7040","position":17,"is_corresponding":false},{"id":254837,"name":"Rosemarie D. Mason","orcid":"0000-0001-7123-4902","position":18,"is_corresponding":false},{"id":105630,"name":"Jason Gorman","orcid":"0000-0002-3775-6560","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-19T02:55:39.010633Z","pmid":"39792559","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}