{"doi":"10.1016/j.celrep.2024.114879","title":"High-throughput identification of calcium-regulated proteins across diverse proteomes","abstract":"Calcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by a lack of high-throughput, unbiased, and quantitative methods to identify protein-calcium engagement. To address this, we adapted protein thermostability assays in budding yeast, human cells, and mouse mitochondria. Based on calcium-dependent thermostability, we identified 2,884 putative calcium-regulated proteins across human, mouse, and yeast proteomes. These data revealed calcium engagement of signaling hubs and cellular processes, including metabolic enzymes and the spliceosome. Cross-species comparison of calcium-protein engagement and mutagenesis experiments identified residue-specific cation engagement, even within well-known EF-hand domains. Additionally, we found that the dienoyl-coenzyme A (CoA) reductase DECR1 binds calcium at physiologically relevant concentrations with substrate-specific affinity, suggesting direct calcium regulation of mitochondrial fatty acid oxidation. These discovery-based proteomic analyses of calcium effectors establish a key resource to dissect cation engagement and its mechanistic effects across multiple species and diverse biological processes.","journal":"Cell Reports","year":2024,"id":428371,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8237,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1209981,"name":"Rose Fields","orcid":"0009-0006-3365-4598","position":1,"is_corresponding":false},{"id":1230030,"name":"Hayden Gizinski","orcid":null,"position":2,"is_corresponding":false},{"id":700669,"name":"George M. Otto","orcid":"0000-0001-9756-1653","position":3,"is_corresponding":false},{"id":417305,"name":"Melissa J. S. MacEwen","orcid":"0000-0002-4852-4626","position":4,"is_corresponding":false},{"id":65936,"name":"Domnița-Valeria Rusnac","orcid":"0000-0001-8828-7701","position":5,"is_corresponding":false},{"id":1229574,"name":"Ping He","orcid":"0000-0002-3751-9610","position":6,"is_corresponding":false},{"id":1209982,"name":"David M Shechner","orcid":"0000-0002-0574-256X","position":7,"is_corresponding":false},{"id":1230031,"name":"Chris D McGann","orcid":null,"position":8,"is_corresponding":false},{"id":719093,"name":"Matthew D. Berg","orcid":"0000-0002-7924-9241","position":9,"is_corresponding":false},{"id":257491,"name":"Judit Villén","orcid":"0000-0002-1005-1739","position":10,"is_corresponding":false},{"id":332382,"name":"Yasemin Sancak","orcid":"0000-0002-9328-7287","position":11,"is_corresponding":false},{"id":107035,"name":"Devin K. Schweppe","orcid":"0000-0002-3241-6276","position":12,"is_corresponding":false},{"id":462947,"name":"Timothy M. Locke","orcid":"0000-0002-9177-7805","position":0,"is_corresponding":true}],"reference_count":102,"raw_metadata":null,"created_at":"2026-07-19T01:58:57.592578Z","pmid":"39425928","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}