{"doi":"10.1016/j.celrep.2024.114874","title":"Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoids","abstract":"Human neural organoid models have become an important tool for studying neurobiology. However, improving the representativeness of neural cell populations in such organoids remains a major effort. In this work, we compared Matrigel, a commercially available matrix, to a neural cadherin (N-cadherin) peptide-functionalized gelatin methacryloyl hydrogel (termed GelMA-Cad) for culturing cortical neural organoids. We determined that peptide presentation can tune cell fate and diversity in gelatin-based matrices during differentiation. Of particular note, cortical organoids cultured in GelMA-Cad hydrogels mapped more closely to human fetal populations and produced neurons with more spontaneous excitatory postsynaptic currents relative to Matrigel. These results provide compelling evidence that matrix-tethered signaling peptides can influence neural organoid differentiation, opening an avenue to control stem cell fate. Moreover, outcomes from this work showcase the technical utility of GelMA-Cad as a simple and defined hydrogel alternative to Matrigel for neural organoid culture.","journal":"Cell Reports","year":2024,"id":420730,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.953,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1212869,"name":"Mia R Haschert","orcid":null,"position":1,"is_corresponding":false},{"id":671186,"name":"José C. Zepeda","orcid":"0000-0002-8045-4883","position":2,"is_corresponding":false},{"id":403441,"name":"Aaron B. Simmons","orcid":null,"position":3,"is_corresponding":false},{"id":1094348,"name":"A K Yates","orcid":"0000-0003-3539-3994","position":4,"is_corresponding":false},{"id":1212299,"name":"Daniel Chavarría","orcid":"0000-0002-6042-2825","position":5,"is_corresponding":false},{"id":1212870,"name":"Melanie Fernandez","orcid":null,"position":6,"is_corresponding":false},{"id":288313,"name":"Gabriella L. Robertson","orcid":"0000-0002-8729-8334","position":7,"is_corresponding":false},{"id":1212300,"name":"Adam M Abdulrahman","orcid":"0000-0002-6424-3162","position":8,"is_corresponding":false},{"id":667642,"name":"Hyosung Kim","orcid":"0000-0003-3568-6500","position":9,"is_corresponding":false},{"id":742577,"name":"Nicole T. Marguerite","orcid":null,"position":10,"is_corresponding":false},{"id":1212871,"name":"Rachel K Moen","orcid":null,"position":11,"is_corresponding":false},{"id":647993,"name":"Lauren E. Drake","orcid":"0000-0003-1067-479X","position":12,"is_corresponding":false},{"id":1175900,"name":"Corinne W. Curry","orcid":null,"position":13,"is_corresponding":false},{"id":667640,"name":"Brian J. O’Grady","orcid":"0000-0002-4648-5096","position":14,"is_corresponding":false},{"id":288316,"name":"Vivian Gama","orcid":"0000-0002-1201-1394","position":15,"is_corresponding":false},{"id":60556,"name":"Ken S. Lau","orcid":"0000-0001-8438-0319","position":16,"is_corresponding":false},{"id":418433,"name":"Brad A. Grueter","orcid":"0000-0002-4224-3866","position":17,"is_corresponding":false},{"id":306019,"name":"Jonathan M. Brunger","orcid":"0000-0001-7468-7982","position":18,"is_corresponding":false},{"id":331785,"name":"Ethan S. Lippmann","orcid":"0000-0001-5703-5747","position":19,"is_corresponding":false},{"id":1094349,"name":"Andrew Kjar","orcid":"0000-0001-5402-8752","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T01:57:31.041511Z","pmid":"39423129","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}