{"doi":"10.1016/j.celrep.2024.114777","title":"A comparative roadmap of PIWI-interacting RNAs across seven species reveals insights into de novo piRNA-precursor formation in mammals","abstract":"PIWI-interacting RNAs (piRNAs) play a crucial role in safeguarding genome integrity by silencing mobile genetic elements. From flies to humans, piRNAs originate from long single-stranded precursors encoded by genomic piRNA clusters. How piRNA clusters form to adapt to genomic invaders and evolve to maintain protection remain key outstanding questions. Here, we generate a roadmap of piRNA clusters across seven species that highlights both similarities and variations. In mammals, we identify transcriptional readthrough as a mechanism to generate piRNAs from transposon insertions (piCs) downstream of genes (DoG). Together with the well-known stress-dependent DoG transcripts, our findings suggest a molecular mechanism for the formation of piRNA clusters in response to retroviral invasion. Finally, we identify a class of dynamic piRNA clusters in humans, underscoring unique features of human germ cell biology. Our results advance the understanding of conserved principles and species-specific variations in piRNA biology and provide tools for future studies.","journal":"Cell Reports","year":2024,"id":432466,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9196,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1041551,"name":"Zuzana Loubalová","orcid":"0000-0002-8876-4456","position":1,"is_corresponding":false},{"id":1237460,"name":"Franziska Ahrend","orcid":null,"position":2,"is_corresponding":false},{"id":1237035,"name":"Aleksandr Friman","orcid":"0000-0002-7968-8455","position":3,"is_corresponding":false},{"id":575777,"name":"Miguel Vasconcelos Almeida","orcid":"0000-0002-4816-3111","position":4,"is_corresponding":false},{"id":465328,"name":"Axel Poulet","orcid":"0000-0002-3415-8960","position":5,"is_corresponding":false},{"id":1042730,"name":"Filip Horvat","orcid":"0000-0002-1896-7645","position":6,"is_corresponding":false},{"id":1237036,"name":"Yuejun Wang","orcid":"0000-0002-4336-0255","position":7,"is_corresponding":false},{"id":345058,"name":"Wolfgang Losert","orcid":"0000-0002-1792-7860","position":8,"is_corresponding":false},{"id":287780,"name":"Hernán Lorenzi","orcid":"0000-0003-0910-7894","position":9,"is_corresponding":false},{"id":1228193,"name":"Petr Svoboda","orcid":"0000-0002-4370-3705","position":10,"is_corresponding":false},{"id":304354,"name":"Eric A. Miska","orcid":"0000-0002-4450-576X","position":11,"is_corresponding":false},{"id":671898,"name":"Josien C. van Wolfswinkel","orcid":"0000-0003-4221-3218","position":12,"is_corresponding":false},{"id":395753,"name":"Astrid D. Haase","orcid":"0000-0003-1120-9013","position":13,"is_corresponding":false},{"id":804536,"name":"Παρθένα Κωνσταντινίδου","orcid":"0009-0005-0492-4117","position":0,"is_corresponding":true}],"reference_count":110,"raw_metadata":null,"created_at":"2026-07-19T01:59:35.267142Z","pmid":"39302833","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}