{"doi":"10.1016/j.celrep.2024.114503","title":"Synaptic plasticity in human thalamocortical assembloids","abstract":"Synaptic plasticities, such as long-term potentiation (LTP) and depression (LTD), tune synaptic efficacy and are essential for learning and memory. Current studies of synaptic plasticity in humans are limited by a lack of adequate human models. Here, we modeled the thalamocortical system by fusing human induced pluripotent stem cell-derived thalamic and cortical organoids. Single-nucleus RNA sequencing revealed that >80% of cells in thalamic organoids were glutamatergic neurons. When fused to form thalamocortical assembloids, thalamic and cortical organoids formed reciprocal long-range axonal projections and reciprocal synapses detectable by light and electron microscopy, respectively. Using whole-cell patch-clamp electrophysiology and two-photon imaging, we characterized glutamatergic synaptic transmission. Thalamocortical and corticothalamic synapses displayed short-term plasticity analogous to that in animal models. LTP and LTD were reliably induced at both synapses; however, their mechanisms differed from those previously described in rodents. Thus, thalamocortical assembloids provide a model system for exploring synaptic plasticity in human circuits.","journal":"Cell Reports","year":2024,"id":419790,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9425,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":659301,"name":"Kristen T. Thomas","orcid":null,"position":1,"is_corresponding":false},{"id":1210492,"name":"Ildar T. Bayazitov","orcid":null,"position":2,"is_corresponding":false},{"id":1210493,"name":"Kyle D. Newman","orcid":null,"position":3,"is_corresponding":false},{"id":1210494,"name":"Nathaniel B. Kurtz","orcid":null,"position":4,"is_corresponding":false},{"id":267294,"name":"Camenzind G. Robinson","orcid":"0000-0002-7277-692X","position":5,"is_corresponding":false},{"id":577340,"name":"Cody A. Ramirez","orcid":"0000-0001-5598-0876","position":6,"is_corresponding":false},{"id":515873,"name":"Alexandra J. Trevisan","orcid":null,"position":7,"is_corresponding":false},{"id":734587,"name":"Jay B. Bikoff","orcid":null,"position":8,"is_corresponding":false},{"id":491670,"name":"Samuel T. Peters","orcid":"0000-0003-1479-8087","position":9,"is_corresponding":false},{"id":250571,"name":"Shondra M. Pruett‐Miller","orcid":"0000-0002-3793-585X","position":10,"is_corresponding":false},{"id":674767,"name":"Yanbo Jiang","orcid":null,"position":11,"is_corresponding":false},{"id":1209903,"name":"A Schild","orcid":"0009-0008-8552-1986","position":12,"is_corresponding":false},{"id":389385,"name":"Anjana Nityanandam","orcid":null,"position":13,"is_corresponding":false},{"id":273882,"name":"Stanislav S. Zakharenko","orcid":"0000-0001-8115-202X","position":14,"is_corresponding":false},{"id":674193,"name":"Mary H. Patton","orcid":"0000-0001-9319-7815","position":0,"is_corresponding":true}],"reference_count":151,"raw_metadata":null,"created_at":"2026-07-19T01:57:19.937291Z","pmid":"39018245","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}