{"doi":"10.1016/j.celrep.2024.114332","title":"A spatiotemporal map of co-receptor signaling networks underlying B cell activation","abstract":"The B cell receptor (BCR) signals together with a multi-component co-receptor complex to initiate B cell activation in response to antigen binding. Here, we take advantage of peroxidase-catalyzed proximity labeling combined with quantitative mass spectrometry to track co-receptor signaling dynamics in Raji cells from 10 s to 2 h after BCR stimulation. This approach enables tracking of 2,814 proximity-labeled proteins and 1,394 phosphosites and provides an unbiased and quantitative molecular map of proteins recruited to the vicinity of CD19, the signaling subunit of the co-receptor complex. We detail the recruitment kinetics of signaling effectors to CD19 and identify previously uncharacterized mediators of B cell activation. We show that the glutamate transporter SLC1A1 is responsible for mediating rapid metabolic reprogramming and for maintaining redox homeostasis during B cell activation. This study provides a comprehensive map of BCR signaling and a rich resource for uncovering the complex signaling networks that regulate activation.","journal":"Cell Reports","year":2024,"id":430417,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9446,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":658552,"name":"Gary A. Bradshaw","orcid":"0000-0001-9724-6100","position":1,"is_corresponding":false},{"id":862956,"name":"Robyn J. Eisert","orcid":"0000-0001-7977-249X","position":2,"is_corresponding":false},{"id":1171432,"name":"Charlotte M. Schilling","orcid":"0000-0003-0132-6777","position":3,"is_corresponding":false},{"id":107033,"name":"Marian Kalocsay","orcid":"0000-0002-4187-5829","position":4,"is_corresponding":false},{"id":852,"name":"Stephen C. Blacklow","orcid":"0000-0002-6904-1981","position":5,"is_corresponding":false},{"id":239851,"name":"Andrew C. Kruse","orcid":"0000-0002-1467-1222","position":6,"is_corresponding":false},{"id":314020,"name":"Katherine J. Susa","orcid":"0000-0003-0077-667X","position":0,"is_corresponding":true}],"reference_count":85,"raw_metadata":null,"created_at":"2026-07-19T01:59:16.092672Z","pmid":"38850533","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}