{"doi":"10.1016/j.celrep.2024.113948","title":"Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution","abstract":"Identifying individual functional B cell receptors (BCRs) is common, but two-dimensional analysis of B cell frequency versus BCR potency would delineate both quantity and quality of antigen-specific memory B cells. We efficiently determine quantitative BCR neutralizing activities using a single-cell-derived antibody supernatant analysis (SCAN) workflow and develop a frequency-potency algorithm to estimate B cell frequencies at various neutralizing activity or binding affinity cutoffs. In an HIV-1 fusion peptide (FP) immunization study, frequency-potency curves elucidate the quantity and quality of FP-specific immunoglobulin G (IgG)+ memory B cells for different animals, time points, and antibody lineages at single-cell resolution. The BCR neutralizing activities are mainly determined by their affinities to soluble envelope trimer. Frequency analysis definitively demonstrates dominant neutralizing antibody lineages. These findings establish SCAN and frequency-potency analyses as promising approaches for general B cell analysis and monoclonal antibody (mAb) discovery. They also provide specific rationales for HIV-1 FP-directed vaccine optimization.","journal":"Cell Reports","year":2024,"id":476486,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9554,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1314439,"name":"Seo-Ho Oh","orcid":null,"position":1,"is_corresponding":false},{"id":1314440,"name":"Catherine S. Yang","orcid":null,"position":2,"is_corresponding":false},{"id":1314023,"name":"Hardik K. Nariya","orcid":"0000-0003-3601-1927","position":3,"is_corresponding":false},{"id":1244543,"name":"Amanda Metz","orcid":"0009-0008-4819-0903","position":4,"is_corresponding":false},{"id":106175,"name":"Amit A. Upadhyay","orcid":"0000-0002-0952-4164","position":5,"is_corresponding":false},{"id":1314441,"name":"Dedeepya R. Gudipati","orcid":null,"position":6,"is_corresponding":false},{"id":997868,"name":"Lizheng Guo","orcid":null,"position":7,"is_corresponding":false},{"id":1314442,"name":"Emily G. McGhee","orcid":null,"position":8,"is_corresponding":false},{"id":611745,"name":"Kiran Gill","orcid":null,"position":9,"is_corresponding":false},{"id":244965,"name":"Elise G. Viox","orcid":"0000-0002-2788-4585","position":10,"is_corresponding":false},{"id":254837,"name":"Rosemarie D. Mason","orcid":"0000-0001-7123-4902","position":11,"is_corresponding":false},{"id":105605,"name":"Nicole A. Doria‐Rose","orcid":"0000-0002-5731-3054","position":12,"is_corresponding":false},{"id":503362,"name":"Kathryn E. Foulds","orcid":"0000-0003-4418-6495","position":13,"is_corresponding":false},{"id":105614,"name":"John R. Mascola","orcid":"0000-0002-5293-4695","position":14,"is_corresponding":false},{"id":273149,"name":"Yuhong Du","orcid":null,"position":15,"is_corresponding":false},{"id":254877,"name":"Haian Fu","orcid":"0000-0002-2362-7979","position":16,"is_corresponding":false},{"id":680026,"name":"John D. Altman","orcid":"0000-0001-9733-2359","position":17,"is_corresponding":false},{"id":263117,"name":"Qi Yan","orcid":"0000-0002-5236-9673","position":18,"is_corresponding":false},{"id":105636,"name":"Zizhang Sheng","orcid":"0000-0002-3253-3309","position":19,"is_corresponding":false},{"id":232942,"name":"Steven E. Bosinger","orcid":"0000-0002-2116-5061","position":20,"is_corresponding":false},{"id":323753,"name":"Rui Kong","orcid":"0000-0003-1779-5885","position":21,"is_corresponding":false},{"id":531728,"name":"Michelle K. Tenggara","orcid":"0000-0001-5582-7775","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T02:06:29.605760Z","pmid":"38483908","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}