{"doi":"10.1016/j.celrep.2024.113940","title":"Co-imaging of RelA and c-Rel reveals features of NF-κB signaling for ligand discrimination","abstract":"Individual cell sensing of external cues has evolved through the temporal patterns in signaling. Since nuclear factor κB (NF-κB) signaling dynamics have been examined using a single subunit, RelA, it remains unclear whether more information might be transmitted via other subunits. Using NF-κB double-knockin reporter mice, we monitored both canonical NF-κB subunits, RelA and c-Rel, simultaneously in single macrophages by quantitative live-cell imaging. We show that signaling features of RelA and c-Rel convey more information about the stimuli than those of either subunit alone. Machine learning is used to predict the ligand identity accurately based on RelA and c-Rel signaling features without considering the co-activated factors. Ligand discrimination is achieved through selective non-redundancy of RelA and c-Rel signaling dynamics, as well as their temporal coordination. These results suggest a potential role of c-Rel in fine-tuning immune responses and highlight the need for approaches that will elucidate the mechanisms regulating NF-κB subunit specificity.","journal":"Cell Reports","year":2024,"id":430990,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1076621,"name":"Apeksha Singh","orcid":"0000-0001-6523-0614","position":1,"is_corresponding":false},{"id":1234802,"name":"S. W. Lowe","orcid":null,"position":2,"is_corresponding":false},{"id":567624,"name":"Mohammad Aqdas","orcid":"0000-0002-3534-0676","position":3,"is_corresponding":false},{"id":398016,"name":"Kevin Jiang","orcid":"0000-0002-1236-6160","position":4,"is_corresponding":false},{"id":576331,"name":"Haripriya Vaidehi Narayanan","orcid":"0000-0001-8220-0724","position":5,"is_corresponding":false},{"id":304204,"name":"Alexander Hoffmann","orcid":"0000-0002-5607-3845","position":6,"is_corresponding":false},{"id":463692,"name":"Myong‐Hee Sung","orcid":"0000-0002-9739-8558","position":7,"is_corresponding":false},{"id":856215,"name":"Shah Md Toufiqur Rahman","orcid":"0000-0001-5419-0696","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T01:59:20.861374Z","pmid":"38483906","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}