{"doi":"10.1016/j.celrep.2023.113534","title":"SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns","abstract":null,"journal":"Cell Reports","year":2023,"id":613915,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1581765,"name":"Luca Sperotto","orcid":null,"position":1,"is_corresponding":false},{"id":1581766,"name":"Stefanie Seuß","orcid":null,"position":2,"is_corresponding":false},{"id":590142,"name":"Hyun-Seo Kang","orcid":"0000-0003-4029-5096","position":3,"is_corresponding":false},{"id":715819,"name":"Rei Yoshimoto","orcid":"0000-0001-6620-8996","position":4,"is_corresponding":false},{"id":3373,"name":"Michael Sattler","orcid":"0000-0002-1594-0527","position":5,"is_corresponding":false},{"id":715820,"name":"Akila Mayeda","orcid":"0000-0002-9562-550X","position":6,"is_corresponding":false},{"id":1581764,"name":"Kazuhiro Fukumura","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns","abstract":"Human pre-mRNA splicing requires the removal of introns with highly variable lengths, from tens to over a million nucleotides. Therefore, mechanisms of intron recognition and splicing are likely not universal. Recently, we reported that splicing in a subset of human short introns with truncated polypyrimidine tracts depends on RBM17 (SPF45), instead of the canonical splicing factor U2 auxiliary factor (U2AF) heterodimer. Here, we demonstrate that SAP30BP, a factor previously implicated in transcriptional control, is an essential splicing cofactor for RBM17. In vitro binding and nuclear magnetic resonance analyses demonstrate that a U2AF-homology motif (UHM) in RBM17 binds directly to a newly identified UHM-ligand motif in SAP30BP. We show that this RBM17-SAP30BP interaction is required to specifically recruit RBM17 to phosphorylated SF3B1 (SF3b155), a U2 small nuclear ribonucleoprotein (U2 snRNP) component in active spliceosomes. We propose a mechanism for splicing in a subset of short introns, in which SAP30BP guides RBM17 in the assembly of active spliceosomes.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38065098","pmcid":null,"openalex_id":"https://openalex.org/W4389433811","authors":[],"funders":[],"total_grants":0,"fwci":1.0207,"citation_percentile":0.74874448,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":3},{"year":2026,"count":5}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"http://www.cell.com/article/S2211124723015462/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S2211124723015462/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2211124723015462?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2211124723015462?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.celrep.2023.113534","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38065098","host_type":"repository"},{"url":"https://push-zb.helmholtz-munich.de/frontdoor.php?source_opus=68933","host_type":"repository"},{"url":"https://push-zb.helmholtz-munich.de/deliver.php?id=38683","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA modifications and cancer","RNA and protein synthesis mechanisms"],"mesh_terms":["RNA Splicing Factors","Splicing Factor U2AF","Humans","Introns","Nuclear Proteins","RNA Precursors","RNA Splicing","Transcription Factors","Spliceosomes","Ribonucleoprotein, U2 Small Nuclear"],"keywords":["RNA splicing","Intron","Group II intron","Genetics","Biology","Computational biology","Alternative splicing","Exon","Gene","RNA","Pre-mrna Splicing","Ulm","Sf3b1","Polypyrimidine Tract","U2af Heterodimer","Uhm","Spf45","Cp: Molecular Biology","U2 Snrnp","Rbm17","Short Intron","Uhm-ligand Motif","Sab30bp","U2af-homology Motif","Sf3b155"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T09:41:13.058966Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}