{"doi":"10.1016/j.celrep.2023.113481","title":"Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system","abstract":"Hydrogen sulfide (H 2 S) is a gaseous microbial metabolite whose role in gut diseases is debated, with contradictory results stemming from experimental difficulties associated with accurate dosing and measuring H 2 S and the use of model systems that do not accurately represent the human gut environment. Here, we engineer Escherichia coli to titrate H 2 S across the physiological range in a gut microphysiological system (chip) supportive of the co-culture of microbes and host cells. The chip is engineered to maintain H 2 S gas tension and enables visualization of co-culture in real time with confocal microscopy. Engineered strains colonize the chip and are metabolically active for 2 days, during which they produce H 2 S across a 16-fold range and induce changes in host gene expression and metabolism in an H 2 S-concentration-dependent manner. These results validate a platform for studying the mechanisms underlying microbe-host interactions by enabling experiments that are infeasible with current animal and in vitro models.","journal":"Cell Reports","year":2023,"id":327831,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9597,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1049222,"name":"Anna W. Lunger","orcid":null,"position":1,"is_corresponding":false},{"id":1049223,"name":"Aayushi S. Sharma","orcid":null,"position":2,"is_corresponding":false},{"id":1037451,"name":"Matthew T. Fernez","orcid":null,"position":3,"is_corresponding":false},{"id":253831,"name":"Rebecca L. Carrier","orcid":"0000-0003-3002-7098","position":4,"is_corresponding":false},{"id":351719,"name":"Abigail N. Koppes","orcid":"0000-0003-0433-9290","position":5,"is_corresponding":false},{"id":351718,"name":"Ryan A. Koppes","orcid":"0000-0002-3376-6358","position":6,"is_corresponding":false},{"id":808354,"name":"Benjamin M. Woolston","orcid":null,"position":7,"is_corresponding":false},{"id":1048640,"name":"Justin A. Hayes","orcid":"0000-0003-2531-8333","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-19T01:08:47.581262Z","pmid":"37980564","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}