{"doi":"10.1016/j.celrep.2023.113392","title":"Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer’s disease","abstract":"The blood-brain barrier (BBB) is primarily manifested by a variety of physiological properties of brain endothelial cells (ECs), but the molecular foundation for these properties remains incompletely clear. Here, we generate a comprehensive molecular atlas of adult brain ECs using acutely purified mouse ECs and integrated multi-omics. Using RNA sequencing (RNA-seq) and proteomics, we identify the transcripts and proteins selectively enriched in brain ECs and demonstrate that they are partially correlated. Using single-cell RNA-seq, we dissect the molecular basis of functional heterogeneity of brain ECs. Using integrative epigenomics and transcriptomics, we determine that TCF/LEF, SOX, and ETS families are top-ranked transcription factors regulating the BBB. We then validate the identified brain-EC-enriched proteins and transcription factors in normal mouse and human brain tissue and assess their expression changes in mice with Alzheimer's disease. Overall, we present a valuable resource with broad implications for regulation of the BBB and treatment of neurological disorders.","journal":"Cell Reports","year":2023,"id":324839,"datarank":0.8828265519598693,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"self_citation_contribution":0.5289540786924243,"citation_network_contribution":0.3538724732674449,"self_endowment_contribution":0.5289540786924243,"citer_contribution":0.3538724732674449,"corpus_percentile":77.12539645702793,"corpus_rank":2958,"citation_count":33,"citer_count":28,"citers_with_citation_signal":23,"citers_with_endowment":23,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.5394,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1042310,"name":"Yage Nie","orcid":null,"position":1,"is_corresponding":false},{"id":1042311,"name":"Jiawen Yang","orcid":null,"position":2,"is_corresponding":false},{"id":1041656,"name":"Shilun Yang","orcid":"0000-0003-2734-3698","position":3,"is_corresponding":false},{"id":283145,"name":"Rui Li","orcid":"0000-0003-0056-7210","position":4,"is_corresponding":false},{"id":324400,"name":"Varsha Rao","orcid":"0000-0002-7348-526X","position":5,"is_corresponding":false},{"id":1041657,"name":"Xiaoyan Hu","orcid":"0000-0002-5751-5668","position":6,"is_corresponding":false},{"id":1041658,"name":"Cheng Fang","orcid":"0009-0000-9684-4870","position":7,"is_corresponding":false},{"id":1041659,"name":"Simeng Li","orcid":"0000-0002-9708-0400","position":8,"is_corresponding":false},{"id":1041660,"name":"Dengpan Song","orcid":"0000-0001-5240-7601","position":9,"is_corresponding":false},{"id":1041661,"name":"Fuyou Guo","orcid":"0000-0001-9332-8272","position":10,"is_corresponding":false},{"id":5648,"name":"Michael P. Snyder","orcid":"0000-0003-0784-7987","position":11,"is_corresponding":false},{"id":26,"name":"Howard Y. Chang","orcid":"0000-0002-9459-4393","position":12,"is_corresponding":false},{"id":228603,"name":"Calvin J. Kuo","orcid":"0000-0002-7427-5985","position":13,"is_corresponding":false},{"id":554486,"name":"Jin Xu","orcid":"0000-0003-0944-9835","position":14,"is_corresponding":false},{"id":602107,"name":"Junlei Chang","orcid":"0000-0002-0319-9022","position":15,"is_corresponding":false},{"id":1041655,"name":"Min Yu","orcid":"0000-0002-4885-8829","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T01:08:16.809511Z","pmid":"37925638","pmcid":"PMC10843806","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}