{"doi":"10.1016/j.celrep.2023.113232","title":"Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes","abstract":"TRPM7 (transient receptor potential cation channel subfamily M member 7) is a chanzyme with channel and kinase domains essential for embryo development. Using gamete-specific Trpm7 -null lines, we report that TRPM7-mediated Mg 2+ influx is indispensable for reaching the blastocyst stage. TRPM7 is expressed dynamically from gametes to blastocysts; displays stage-specific localization on the plasma membrane, cytoplasm, and nucleus; and undergoes cleavage that produces C-terminal kinase fragments. TRPM7 underpins Mg 2+ homeostasis, and excess Mg 2+ but not Zn 2+ or Ca 2+ overcomes the arrest of Trpm7 -null embryos; expressing Trpm7 mRNA restores development, but mutant versions fail or are partially rescued. Transcriptomic analyses of Trpm7 -null embryos reveal an abundance of oxidative stress-pathway genes, confirmed by mitochondrial dysfunction, and a reduction in transcription factor networks essential for proliferation; Mg 2+ supplementation corrects these defects. Hence, TRPM7 underpins Mg 2+ homeostasis in preimplantation embryos, prevents oxidative stress, and promotes gene expression patterns necessary for developmental progression and cell-lineage specification.","journal":"Cell Reports","year":2023,"id":341738,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1077752,"name":"Cristina Soriano‐Úbeda","orcid":"0000-0003-2880-3342","position":1,"is_corresponding":false},{"id":310695,"name":"Paula Stein","orcid":"0000-0002-2040-4593","position":2,"is_corresponding":false},{"id":310696,"name":"Virginia Savy","orcid":"0000-0002-4942-1338","position":3,"is_corresponding":false},{"id":320761,"name":"Brian N. Papas","orcid":"0000-0003-4540-1142","position":4,"is_corresponding":false},{"id":371829,"name":"Goli Ardestani","orcid":"0000-0002-2599-5361","position":5,"is_corresponding":false},{"id":371831,"name":"Ingrid Carvacho","orcid":"0000-0003-2311-7239","position":6,"is_corresponding":false},{"id":380770,"name":"Dominique Alfandari","orcid":"0000-0002-0557-1246","position":7,"is_corresponding":false},{"id":310698,"name":"Carmen J. Williams","orcid":"0000-0001-6440-7086","position":8,"is_corresponding":false},{"id":371832,"name":"Rafael A. Fissore","orcid":"0000-0001-5655-0915","position":9,"is_corresponding":false},{"id":928690,"name":"Neha Gupta","orcid":"0000-0003-4055-8167","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":null,"created_at":"2026-07-19T01:11:08.077724Z","pmid":"37824328","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}