{"doi":"10.1016/j.celrep.2023.113136","title":"Principles of assembly and regulation of condensates of Polycomb repressive complex 1 through phase separation","abstract":"Polycomb repressive complex 1 (PRC1) undergoes phase separation to form Polycomb condensates that are multi-component hubs for silencing Polycomb target genes. In this study, we demonstrate that formation and regulation of PRC1 condensates are consistent with the scaffold-client model, where the Chromobox 2 (CBX2) protein behaves as the scaffold while the other PRC1 proteins are clients. Such clients induce a re-entrant phase transition of CBX2 condensates. The composition of the multi-component PRC1 condensates (1) determines the dynamic properties of the scaffold protein; (2) selectively promotes the formation of CBX4-PRC1 condensates while dissolving condensates of CBX6-, CBX7-, and CBX8-PRC1; and (3) controls the enrichment of CBX4-, CBX7-, and CBX8-PRC1 in CBX2-PRC1 condensates and the exclusion of CBX6-PRC1 from CBX2-PRC1 condensates. Our findings uncover how multi-component PRC1 condensates are assembled via an intricate scaffold-client mechanism whereby the properties of the PRC1 condensates are sensitively regulated by its composition and stoichiometry.","journal":"Cell Reports","year":2023,"id":322835,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9548,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1037029,"name":"Pin Yu Chew","orcid":"0000-0002-6401-6154","position":1,"is_corresponding":false},{"id":667042,"name":"Steven Ingersoll","orcid":null,"position":2,"is_corresponding":false},{"id":1037030,"name":"Jorge R. Espinosa","orcid":"0000-0001-9530-2658","position":3,"is_corresponding":false},{"id":1037031,"name":"Anne Aguirre","orcid":"0000-0001-6974-0291","position":4,"is_corresponding":false},{"id":1037534,"name":"Axel Espinoza","orcid":null,"position":5,"is_corresponding":false},{"id":1037535,"name":"Joey Wen","orcid":null,"position":6,"is_corresponding":false},{"id":1037536,"name":"Kalkidan Astatike","orcid":null,"position":7,"is_corresponding":false},{"id":314067,"name":"Tatiana G. Kutateladze","orcid":"0000-0001-7375-6990","position":8,"is_corresponding":false},{"id":1037032,"name":"Rosana Collepardo‐Guevara","orcid":"0000-0003-1781-7351","position":9,"is_corresponding":false},{"id":264392,"name":"Xiaojun Ren","orcid":"0000-0002-3931-7625","position":10,"is_corresponding":false},{"id":264388,"name":"Kyle Brown","orcid":"0000-0001-9640-6520","position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T01:07:47.432636Z","pmid":"37756159","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}