{"doi":"10.1016/j.celrep.2023.112005","title":"CDK1 bridges NF-κB and β-catenin signaling in response to H. pylori infection in gastric tumorigenesis","abstract":"Infection with Helicobacter pylori (H. pylori) is the main risk factor for gastric cancer, a leading cause of cancer-related death worldwide. The oncogenic functions of cyclin-dependent kinase 1 (CDK1) are not fully understood in gastric tumorigenesis. Using public datasets, quantitative real-time PCR, western blot, and immunohistochemical (IHC) analyses, we detect high levels of CDK1 in human and mouse gastric tumors. H. pylori infection induces activation of nuclear factor κB (NF-κB) with a significant increase in CDK1 in in vitro and in vivo models (p < 0.01). We confirm active NF-κB binding sites on the CDK1 promoter sequence. CDK1 phosphorylates and inhibits GSK-3β activity through direct binding with subsequent accumulation and activation of β-catenin. CDK1 silencing or pharmacologic inhibition reverses these effects and impairs tumor organoids and spheroid formation. IHC analysis demonstrates a positive correlation between CDK1 and β-catenin. The results demonstrate a mechanistic link between infection, inflammation, and gastric tumorigenesis where CDK1 plays a critical role.","journal":"Cell Reports","year":2023,"id":322342,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":40,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9576,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":930404,"name":"Marwah M. Al‐Mathkour","orcid":null,"position":1,"is_corresponding":false},{"id":809894,"name":"Long‐Long Cao","orcid":null,"position":2,"is_corresponding":false},{"id":426780,"name":"Shayan Khalafi","orcid":"0000-0001-5208-0581","position":3,"is_corresponding":false},{"id":426781,"name":"Zheng Chen","orcid":"0000-0002-6582-847X","position":4,"is_corresponding":false},{"id":96730,"name":"Julio Poveda","orcid":"0009-0003-3075-0849","position":5,"is_corresponding":false},{"id":426782,"name":"Dunfa Peng","orcid":"0000-0002-2182-7221","position":6,"is_corresponding":false},{"id":426783,"name":"Heng Lu","orcid":"0009-0000-6562-4277","position":7,"is_corresponding":false},{"id":426784,"name":"Mohammed Soutto","orcid":"0000-0002-5597-4588","position":8,"is_corresponding":false},{"id":886319,"name":"Tianling Hu","orcid":null,"position":9,"is_corresponding":false},{"id":96728,"name":"Oliver G. McDonald","orcid":"0000-0002-6602-7535","position":10,"is_corresponding":false},{"id":426786,"name":"Alexander Zaika","orcid":"0000-0001-6760-2020","position":11,"is_corresponding":false},{"id":426787,"name":"Wael El‐Rifai","orcid":"0000-0003-2101-6098","position":12,"is_corresponding":false},{"id":427554,"name":"Shoumin Zhu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-19T01:07:42.497873Z","pmid":"36681899","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}