{"doi":"10.1016/j.celrep.2022.111858","title":"Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes","abstract":"The histone chaperone FACT (facilitates chromatin transcription) enhances transcription in eukaryotic cells, targeting DNA-protein interactions. FACT, a heterodimer in humans, comprises SPT16 and SSRP1 subunits. We measure nucleosome stability and dynamics in the presence of FACT and critical component domains. Optical tweezers quantify FACT/subdomain binding to nucleosomes, displacing the outer wrap of DNA, disrupting direct DNA-histone (core site) interactions, altering the energy landscape of unwrapping, and increasing the kinetics of DNA-histone disruption. Atomic force microscopy reveals nucleosome remodeling, while single-molecule fluorescence quantifies kinetics of histone loss for disrupted nucleosomes, a process accelerated by FACT. Furthermore, two isolated domains exhibit contradictory functions; while the SSRP1 HMGB domain displaces DNA, SPT16 MD/CTD stabilizes DNA-H2A/H2B dimer interactions. However, only intact FACT tethers disrupted DNA to the histones and supports rapid nucleosome reformation over several cycles of force disruption/release. These results demonstrate that key FACT domains combine to catalyze both nucleosome disassembly and reassembly.","journal":"Cell Reports","year":2022,"id":253980,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":890526,"name":"Michael Morse","orcid":"0000-0002-8561-1833","position":1,"is_corresponding":false},{"id":521147,"name":"Nicole A. Becker","orcid":"0000-0002-2904-0909","position":2,"is_corresponding":false},{"id":900500,"name":"Qi Hu","orcid":"0000-0003-3647-8471","position":3,"is_corresponding":false},{"id":347203,"name":"Maria Victoria Botuyan","orcid":"0000-0002-6466-7432","position":4,"is_corresponding":false},{"id":900501,"name":"Emily Navarrete","orcid":"0000-0002-3986-8099","position":5,"is_corresponding":false},{"id":900502,"name":"Ran Huo","orcid":"0000-0001-5166-127X","position":6,"is_corresponding":false},{"id":352667,"name":"Uma M. Muthurajan","orcid":"0000-0003-2596-5848","position":7,"is_corresponding":false},{"id":405365,"name":"Ioulia Rouzina","orcid":"0000-0003-4651-5264","position":8,"is_corresponding":false},{"id":34465,"name":"Karolin Luger","orcid":"0000-0001-5136-5331","position":9,"is_corresponding":false},{"id":347207,"name":"Georges Mer","orcid":"0000-0002-1900-1578","position":10,"is_corresponding":false},{"id":407487,"name":"L. James Maher","orcid":"0000-0002-5043-6422","position":11,"is_corresponding":false},{"id":405368,"name":"Mark C. Williams","orcid":"0000-0003-3219-376X","position":12,"is_corresponding":false},{"id":405364,"name":"Micah J. McCauley","orcid":"0000-0003-2115-2989","position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-19T00:25:03.918844Z","pmid":"36577379","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}