{"doi":"10.1016/j.celrep.2022.111822","title":"A specific type of Argonaute phosphorylation regulates binding to microRNAs during C. elegans development","abstract":"Argonaute proteins are at the core of the microRNA-mediated gene silencing pathway essential for animals. In C. elegans, the microRNA-specific Argonautes ALG-1 and ALG-2 regulate multiple processes required for proper animal developmental timing and viability. Here we identified a phosphorylation site on ALG-1 that modulates microRNA association. Mutating ALG-1 serine 642 into a phospho-mimicking residue impairs microRNA binding and causes embryonic lethality and post-embryonic phenotypes that are consistent with alteration of microRNA functions. Monitoring microRNA levels in alg-1 phosphorylation mutant animals shows that microRNA passenger strands increase in abundance but are not preferentially loaded into ALG-1, indicating that the miRNA binding defects could lead to microRNA duplex accumulation. Our genetic and biochemical experiments support protein kinase A (PKA) KIN-1 as the putative kinase that phosphorylates ALG-1 serine 642. Our data indicate that PKA triggers ALG-1 phosphorylation to regulate its microRNA association during C. elegans development.","journal":"Cell Reports","year":2022,"id":258165,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9567,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":779388,"name":"Vivek Nilesh Shah","orcid":"0000-0002-5547-4604","position":1,"is_corresponding":false},{"id":909235,"name":"Smita Nahar","orcid":"0000-0001-7449-9324","position":2,"is_corresponding":false},{"id":909236,"name":"Julia Neumeier","orcid":"0009-0004-1157-1834","position":3,"is_corresponding":false},{"id":779868,"name":"François Houle","orcid":null,"position":4,"is_corresponding":false},{"id":638798,"name":"Astrid Bruckmann","orcid":"0000-0001-6082-2944","position":5,"is_corresponding":false},{"id":568756,"name":"Foivos Gypas","orcid":"0000-0002-7233-8794","position":6,"is_corresponding":false},{"id":347233,"name":"Kotaro Nakanishi","orcid":"0000-0002-9857-6010","position":7,"is_corresponding":false},{"id":372024,"name":"Helge Großhans","orcid":"0000-0002-8169-6905","position":8,"is_corresponding":false},{"id":623813,"name":"Gunter Meister","orcid":"0000-0002-2098-9923","position":9,"is_corresponding":false},{"id":779392,"name":"Martin J. Simard","orcid":"0000-0002-3189-9309","position":10,"is_corresponding":false},{"id":779390,"name":"Miguel Quévillon Huberdeau","orcid":"0000-0003-4410-906X","position":0,"is_corresponding":true}],"reference_count":123,"raw_metadata":null,"created_at":"2026-07-19T00:25:38.634785Z","pmid":"36516777","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}