{"doi":"10.1016/j.celrep.2022.111682","title":"Double knockin mice show NF-κB trajectories in immune signaling and aging","abstract":"In vitro studies suggest that mapping the spatiotemporal complexity of nuclear factor κB (NF-κB) signaling is essential to understanding its function. The lack of tools to directly monitor NF-κB proteins in vivo has hindered such efforts. Here, we introduce reporter mice with the endogenous RelA (p65) or c-Rel labeled with distinct fluorescent proteins and a double knockin with both subunits labeled. Overcoming hurdles in simultaneous live-cell imaging of RelA and c-Rel, we show that quantitative features of signaling reflect the identity of activating ligands, differ between primary and immortalized cells, and shift toward c-Rel in microglia from aged brains. RelA:c-Rel heterodimer is unexpectedly depleted in the nuclei of stimulated cells. Trajectories of subunit co-expression in immune lineages reveal a reduction at key cell maturation stages. These results demonstrate the power of these reporters in gaining deeper insights into NF-κB biology, with the spectral complementarity of the labeled NF-κB proteins enabling diverse applications.","journal":"Cell Reports","year":2022,"id":242723,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":37,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9462,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":567624,"name":"Mohammad Aqdas","orcid":"0000-0002-3534-0676","position":1,"is_corresponding":false},{"id":463689,"name":"Erik W. Martin","orcid":"0000-0003-3979-2378","position":2,"is_corresponding":false},{"id":275777,"name":"Francesco Tomassoni‐Ardori","orcid":"0000-0002-8824-3693","position":3,"is_corresponding":false},{"id":530000,"name":"Preeyaporn Songkiatisak","orcid":"0000-0002-0205-4673","position":4,"is_corresponding":false},{"id":567622,"name":"Kyu‐Seon Oh","orcid":"0000-0001-6373-7788","position":5,"is_corresponding":false},{"id":598706,"name":"Stefan Uderhardt","orcid":"0000-0002-2489-2109","position":6,"is_corresponding":false},{"id":873857,"name":"Sangwon Yun","orcid":"0000-0002-1156-8094","position":7,"is_corresponding":false},{"id":873858,"name":"Quia Claybourne","orcid":"0000-0003-1056-235X","position":8,"is_corresponding":false},{"id":323570,"name":"Ross A. McDevitt","orcid":"0000-0003-3722-9047","position":9,"is_corresponding":false},{"id":281515,"name":"Valentina Greco","orcid":"0000-0002-6492-4603","position":10,"is_corresponding":false},{"id":228444,"name":"Ronald N. Germain","orcid":"0000-0003-1495-9143","position":11,"is_corresponding":false},{"id":275782,"name":"Lino Tessarollo","orcid":"0000-0001-6420-772X","position":12,"is_corresponding":false},{"id":463692,"name":"Myong‐Hee Sung","orcid":"0000-0002-9739-8558","position":13,"is_corresponding":false},{"id":856215,"name":"Shah Md Toufiqur Rahman","orcid":"0000-0001-5419-0696","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-19T00:23:10.218426Z","pmid":"36417863","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}