{"doi":"10.1016/j.celrep.2022.111096","title":"Pulse labeling reveals the tail end of protein folding by proteome profiling","abstract":"Accurate and efficient folding of nascent protein sequences into their native states requires support from the protein homeostasis network. Herein we probe which newly translated proteins are thermo-sensitive, making them susceptible to misfolding and aggregation under heat stress using pulse-SILAC mass spectrometry. We find a distinct group of proteins that is highly sensitive to this perturbation when newly synthesized but not once matured. These proteins are abundant and highly structured. Notably, they display a tendency to form β sheet secondary structures, have more complex folding topology, and are enriched for chaperone-binding motifs, suggesting a higher demand for chaperone-assisted folding. These polypeptides are also more often components of stable protein complexes in comparison with other proteins. Combining these findings suggests the existence of a specific subset of proteins in the cell that is particularly vulnerable to misfolding and aggregation following synthesis before reaching the native state.","journal":"Cell Reports","year":2022,"id":258875,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":910504,"name":"Erich R. Kuechler","orcid":"0000-0002-4733-7554","position":1,"is_corresponding":false},{"id":910505,"name":"Ryan Wong","orcid":"0000-0002-9339-2677","position":2,"is_corresponding":false},{"id":910506,"name":"Gaetano Calabrese","orcid":"0000-0002-2212-5280","position":3,"is_corresponding":false},{"id":806965,"name":"Ian Sitarik","orcid":"0000-0001-8553-923X","position":4,"is_corresponding":false},{"id":910507,"name":"Viraj Rana","orcid":"0000-0002-0301-3697","position":5,"is_corresponding":false},{"id":122857,"name":"Nikolay Stoynov","orcid":null,"position":6,"is_corresponding":false},{"id":323020,"name":"Edward P. O’Brien","orcid":"0000-0001-9809-3273","position":7,"is_corresponding":false},{"id":292664,"name":"Jörg Gsponer","orcid":"0000-0003-3672-6594","position":8,"is_corresponding":false},{"id":910508,"name":"Thibault Mayor","orcid":"0000-0002-9289-3939","position":9,"is_corresponding":false},{"id":910503,"name":"Mang Zhu","orcid":"0000-0002-3177-5541","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-19T00:25:43.037820Z","pmid":"35858568","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}