{"doi":"10.1016/j.celrep.2022.111050","title":"A single-cell analysis of thymopoiesis and thymic iNKT cell development in pigs","abstract":"Many aspects of the porcine immune system remain poorly characterized, which poses a barrier to improving swine health and utilizing pigs as preclinical models. Here, we employ single-cell RNA sequencing (scRNA-seq) to create a cell atlas of the early-adolescent pig thymus. Our data show conserved features as well as species-specific differences in cell states and cell types compared with human thymocytes. We also describe several unconventional T cell types with gene expression profiles associated with innate effector functions. This includes a cell census of more than 11,000 differentiating invariant natural killer T (iNKT) cells, which reveals that the functional diversity of pig iNKT cells differs substantially from the iNKT0/1/2/17 subset differentiation paradigm established in mice. Our data characterize key differentiation events in porcine thymopoiesis and iNKT cell maturation and provide important insights into pig T cell development.","journal":"Cell Reports","year":2022,"id":244022,"datarank":0.8417142392474853,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.3218538538275263,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.3218538538275263,"corpus_percentile":76.08107062736907,"corpus_rank":3093,"citation_count":31,"citer_count":25,"citers_with_citation_signal":12,"citers_with_endowment":12,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.5386,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":397275,"name":"Darling Mélany de Carvalho Madrid","orcid":"0000-0002-6459-5483","position":1,"is_corresponding":false},{"id":282038,"name":"Sebastian Joyce","orcid":"0000-0002-3183-1451","position":2,"is_corresponding":false},{"id":397274,"name":"John P. Driver","orcid":"0000-0002-6714-3335","position":3,"is_corresponding":false},{"id":398599,"name":"Weihong Gu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":151,"raw_metadata":null,"created_at":"2026-07-19T00:23:25.718788Z","pmid":"35793622","pmcid":"PMC9704770","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}