{"doi":"10.1016/j.celrep.2022.111009","title":"Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike","abstract":"The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron BA.2 sub-lineage has gained in proportion relative to BA.1. Because spike (S) protein variations may underlie differences in their pathobiology, here we determine cryoelectron microscopy (cryo-EM) structures of the BA.2 S ectodomain and compare these with previously determined BA.1 S structures. BA.2 receptor-binding domain (RBD) mutations induce remodeling of the RBD structure, resulting in tighter packing and improved thermostability. Interprotomer RBD interactions are enhanced in the closed (or 3-RBD-down) BA.2 S, while the fusion peptide is less accessible to antibodies than in BA.1. Binding and pseudovirus neutralization assays reveal extensive immune evasion while defining epitopes of two outer RBD face-binding antibodies, DH1044 and DH1193, that neutralize both BA.1 and BA.2. Taken together, our results indicate that stabilization of the closed state through interprotomer RBD-RBD packing is a hallmark of the Omicron variant and show differences in key functional regions in the BA.1 and BA.2 S proteins.","journal":"Cell Reports","year":2022,"id":233991,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":110,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9388,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":422463,"name":"Jared Lindenberger","orcid":"0000-0003-2044-6715","position":1,"is_corresponding":false},{"id":229642,"name":"S. Gobeil","orcid":null,"position":2,"is_corresponding":false},{"id":227357,"name":"Rory Henderson","orcid":"0000-0002-4301-6382","position":3,"is_corresponding":false},{"id":229643,"name":"Rob Parks","orcid":null,"position":4,"is_corresponding":false},{"id":469212,"name":"Maggie Barr","orcid":"0000-0002-0408-0728","position":5,"is_corresponding":false},{"id":553352,"name":"Margaret Deyton","orcid":null,"position":6,"is_corresponding":false},{"id":842177,"name":"Mitchell Martin","orcid":"0000-0002-1043-4465","position":7,"is_corresponding":false},{"id":227360,"name":"Katarzyna Janowska","orcid":"0000-0002-7936-2244","position":8,"is_corresponding":false},{"id":842175,"name":"Xiao Huang","orcid":"0000-0002-5225-3224","position":9,"is_corresponding":false},{"id":842176,"name":"Aaron May","orcid":"0000-0003-2262-9750","position":10,"is_corresponding":false},{"id":843171,"name":"Micah Speakman","orcid":null,"position":11,"is_corresponding":false},{"id":629931,"name":"Esther Beaudoin","orcid":"0000-0003-1813-6531","position":12,"is_corresponding":false},{"id":559052,"name":"Bryan Kraft","orcid":"0000-0001-6545-8803","position":13,"is_corresponding":false},{"id":618515,"name":"Xiaozhi Lu","orcid":"0000-0002-2517-6331","position":14,"is_corresponding":false},{"id":227358,"name":"Robert J. Edwards","orcid":"0000-0003-4446-1194","position":15,"is_corresponding":false},{"id":553750,"name":"Amanda Eaton","orcid":"0000-0003-3064-2947","position":16,"is_corresponding":false},{"id":103755,"name":"David C. Montefiori","orcid":"0000-0003-0856-6319","position":17,"is_corresponding":false},{"id":280544,"name":"Wilton B. Williams","orcid":"0000-0002-2970-7259","position":18,"is_corresponding":false},{"id":230387,"name":"Kevin O. Saunders","orcid":"0000-0001-7399-7954","position":19,"is_corresponding":false},{"id":274230,"name":"Kevin Wiehe","orcid":"0000-0001-9933-9618","position":20,"is_corresponding":false},{"id":96347,"name":"Barton F. Haynes","orcid":"0000-0002-7643-9023","position":21,"is_corresponding":false},{"id":227366,"name":"Priyamvada Acharya","orcid":"0000-0002-0089-277X","position":22,"is_corresponding":false},{"id":227361,"name":"Victoria Stalls","orcid":"0000-0002-1878-0581","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T00:21:31.237180Z","pmid":"35732171","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}