{"doi":"10.1016/j.celrep.2022.110976","title":"Invading viral DNA triggers dsRNA synthesis by RNA polymerase II to activate antiviral RNA interference in Drosophila","abstract":"dsRNA sensing triggers antiviral responses against RNA and DNA viruses in diverse eukaryotes. In Drosophila, Invertebrate iridescent virus 6 (IIV-6), a large DNA virus, triggers production of small interfering RNAs (siRNAs) by the dsRNA sensor Dicer-2. Here, we show that host RNA polymerase II (RNAPII) bidirectionally transcribes specific AT-rich regions of the IIV-6 DNA genome to generate dsRNA. Both replicative and naked IIV-6 genomes trigger production of dsRNA in Drosophila cells, implying direct sensing of invading DNA. Loquacious-PD, a Dicer-2 co-factor essential for the biogenesis of endogenous siRNAs, is dispensable for processing of IIV-6-derived dsRNAs, which suggests that they are distinct. Consistent with this finding, inhibition of the RNAPII co-factor P-TEFb affects the synthesis of endogenous, but not virus-derived, dsRNA. Altogether, our results suggest that a non-canonical RNAPII complex recognizes invading viral DNA to synthesize virus-derived dsRNA, which activates the antiviral siRNA pathway in Drosophila.","journal":"Cell Reports","year":2022,"id":251900,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.954,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":468114,"name":"Eric Roberto Guimarães Rocha Aguiar","orcid":"0000-0002-8143-5756","position":1,"is_corresponding":false},{"id":895354,"name":"Roenick Proveti Olmo","orcid":"0000-0002-3849-8591","position":2,"is_corresponding":false},{"id":895355,"name":"Juliana Alves‐Silva","orcid":"0000-0002-7627-3000","position":3,"is_corresponding":false},{"id":895356,"name":"Laurent Daeffler","orcid":"0000-0001-5871-5082","position":4,"is_corresponding":false},{"id":270184,"name":"Richard W. Carthew","orcid":"0000-0003-0343-0156","position":5,"is_corresponding":false},{"id":624017,"name":"Jean‐Luc Imler","orcid":"0000-0003-0740-8319","position":6,"is_corresponding":false},{"id":468115,"name":"João T. Marques","orcid":"0000-0002-3457-3320","position":7,"is_corresponding":false},{"id":895353,"name":"Isaque J. S. de Faria","orcid":"0000-0002-8810-8133","position":0,"is_corresponding":true}],"reference_count":100,"raw_metadata":null,"created_at":"2026-07-19T00:24:41.993282Z","pmid":"35732126","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}