{"doi":"10.1016/j.celrep.2022.110885","title":"Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model","abstract":"Physiological blood-tissue barriers play a critical role in separating the circulation from immune-privileged sites and denying access to blood-borne viruses. The mechanism of virus restriction by these barriers is poorly understood. We utilize induced pluripotent stem cell (iPSC)-derived human brain microvascular endothelial cells (iBMECs) to study virus-blood-brain barrier (BBB) interactions. These iPSC-derived cells faithfully recapitulate a striking difference in in vivo neuroinvasion by two alphavirus isolates and are selectively permissive to neurotropic flaviviruses. A model of cocultured iBMECs and astrocytes exhibits high transendothelial electrical resistance and blocks non-neurotropic flaviviruses from getting across the barrier. We find that iBMECs constitutively express an interferon-induced gene, IFITM1, which preferentially restricts the replication of non-neurotropic flaviviruses. Barrier cells from blood-testis and blood-retinal barriers also constitutively express IFITMs that contribute to the viral resistance. Our application of a renewable human iPSC-based model for studying virus-BBB interactions reveals that intrinsic immunity at the barriers contributes to virus exclusion.","journal":"Cell Reports","year":2022,"id":254391,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9582,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":901057,"name":"Angelica Medina","orcid":"0009-0002-8471-0212","position":1,"is_corresponding":false},{"id":901058,"name":"Zhenlan Yao","orcid":"0000-0001-6588-0633","position":2,"is_corresponding":false},{"id":901059,"name":"Mausumi Basu","orcid":"0000-0003-1143-2071","position":3,"is_corresponding":false},{"id":255326,"name":"Janhavi P. Natekar","orcid":"0000-0003-0340-082X","position":4,"is_corresponding":false},{"id":901482,"name":"Jianshe Lang","orcid":null,"position":5,"is_corresponding":false},{"id":901060,"name":"Egan Sanchez","orcid":"0000-0003-2340-406X","position":6,"is_corresponding":false},{"id":901483,"name":"Mezindia B. Nkembo","orcid":null,"position":7,"is_corresponding":false},{"id":301308,"name":"Chongchong Xu","orcid":"0000-0002-9867-9220","position":8,"is_corresponding":false},{"id":227202,"name":"Xuyu Qian","orcid":"0000-0001-5944-3816","position":9,"is_corresponding":false},{"id":70047,"name":"Phuong Nguyen","orcid":"0000-0001-6518-4849","position":10,"is_corresponding":false},{"id":227213,"name":"Zhexing Wen","orcid":"0000-0002-1518-3845","position":11,"is_corresponding":false},{"id":123278,"name":"Hongjun Song","orcid":"0000-0002-8720-5310","position":12,"is_corresponding":false},{"id":21533,"name":"Guo‐li Ming","orcid":"0000-0002-2517-6075","position":13,"is_corresponding":false},{"id":255329,"name":"Mukesh Kumar","orcid":"0000-0003-0970-4875","position":14,"is_corresponding":false},{"id":361248,"name":"Margo A. Brinton","orcid":"0000-0003-4868-175X","position":15,"is_corresponding":false},{"id":319806,"name":"Melody M. H. Li","orcid":"0000-0002-6905-7940","position":16,"is_corresponding":false},{"id":313662,"name":"Hengli Tang","orcid":"0000-0002-9358-410X","position":17,"is_corresponding":false},{"id":580292,"name":"Yichen Cheng","orcid":"0000-0003-3127-0102","position":0,"is_corresponding":true}],"reference_count":125,"raw_metadata":null,"created_at":"2026-07-19T00:25:03.918844Z","pmid":"35649379","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}