{"doi":"10.1016/j.celrep.2021.109928","title":"Neutralizing antibody 5-7 defines a distinct site of vulnerability in SARS-CoV-2 spike N-terminal domain","abstract":"Antibodies that potently neutralize SARS-CoV-2 target mainly the receptor-binding domain or the N-terminal domain (NTD). Over a dozen potently neutralizing NTD-directed antibodies have been studied structurally, and all target a single antigenic supersite in NTD (site 1). Here, we report the cryo-EM structure of a potent NTD-directed neutralizing antibody 5-7, which recognizes a site distinct from other potently neutralizing antibodies, inserting a binding loop into an exposed hydrophobic pocket between the two sheets of the NTD β sandwich. Interestingly, this pocket was previously identified as the binding site for hydrophobic molecules, including heme metabolites, but we observe that their presence does not substantially impede 5-7 recognition. Mirroring its distinctive binding, antibody 5-7 retains neutralization potency with many variants of concern (VOCs). Overall, we reveal that a hydrophobic pocket in NTD proposed for immune evasion can be used by the immune system for recognition.","journal":"Cell Reports","year":2021,"id":151702,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":482831,"name":"Yicheng Guo","orcid":"0000-0001-5357-660X","position":1,"is_corresponding":false},{"id":105619,"name":"Pengfei Wang","orcid":"0000-0003-2454-7652","position":2,"is_corresponding":false},{"id":105620,"name":"Manoj S. Nair","orcid":"0000-0002-5994-3957","position":3,"is_corresponding":false},{"id":616934,"name":"Maple Wang","orcid":null,"position":4,"is_corresponding":false},{"id":105637,"name":"Yaoxing Huang","orcid":"0000-0001-6270-1644","position":5,"is_corresponding":false},{"id":105621,"name":"Jian Yu","orcid":"0000-0001-8604-4538","position":6,"is_corresponding":false},{"id":105618,"name":"Lihong Liu","orcid":"0000-0003-3128-8513","position":7,"is_corresponding":false},{"id":229150,"name":"Phinikoula S. Katsamba","orcid":"0000-0003-3981-1604","position":8,"is_corresponding":false},{"id":327783,"name":"Fabiana Bahna","orcid":null,"position":9,"is_corresponding":false},{"id":527381,"name":"Eswar R. Reddem","orcid":"0000-0003-3054-5506","position":10,"is_corresponding":false},{"id":105633,"name":"Peter D. Kwong","orcid":"0000-0003-3560-232X","position":11,"is_corresponding":false},{"id":104013,"name":"David D. Ho","orcid":"0000-0003-1627-149X","position":12,"is_corresponding":false},{"id":105636,"name":"Zizhang Sheng","orcid":"0000-0002-3253-3309","position":13,"is_corresponding":false},{"id":105638,"name":"Lawrence Shapiro","orcid":"0000-0001-9943-8819","position":14,"is_corresponding":false},{"id":105628,"name":"Gabriele Cerutti","orcid":"0000-0002-9081-7561","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-18T23:43:16.823357Z","pmid":"34706271","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}