{"doi":"10.1016/j.celrep.2021.109846","title":"Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart","abstract":"Optical methods for measuring intracellular ions including Ca 2+ revolutionized our understanding of signal transduction. However, these methods are not extensively applied to intact organs due to issues including inner filter effects, motion, and available probes. Mitochondrial Ca 2+ is postulated to regulate cell energetics and death pathways that are best studied in an intact organ. Here, we develop a method to optically measure mitochondrial Ca 2+ and demonstrate its validity for mitochondrial Ca 2+ and metabolism using hearts from wild-type mice and mice with germline knockout of the mitochondria calcium uniporter (MCU-KO). We previously reported that germline MCU-KO hearts do not show an impaired response to adrenergic stimulation. We find that these MCU-KO hearts do not take up Ca 2+ , consistent with no alternative Ca 2+ uptake mechanisms in the absence of MCU. This approach can address the role of mitochondrial Ca 2+ to the myriad of functions attributed to alterations in mitochondrial Ca 2+ .","journal":"Cell Reports","year":2021,"id":163421,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9467,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":483231,"name":"Bárbara Román","orcid":"0000-0003-2400-2177","position":1,"is_corresponding":false},{"id":461835,"name":"Junhui Sun","orcid":"0000-0002-1816-1190","position":2,"is_corresponding":false},{"id":684076,"name":"Armel N. Femnou","orcid":null,"position":3,"is_corresponding":false},{"id":683161,"name":"Fan Zhang","orcid":"0000-0002-0744-673X","position":4,"is_corresponding":false},{"id":242699,"name":"Chengyu Liu","orcid":"0000-0002-5136-9225","position":5,"is_corresponding":false},{"id":552771,"name":"Christian A. Combs","orcid":"0000-0003-4656-4840","position":6,"is_corresponding":false},{"id":675219,"name":"Robert S. Balaban","orcid":"0000-0003-4086-0948","position":7,"is_corresponding":false},{"id":228250,"name":"Elizabeth Murphy","orcid":"0000-0001-9400-0797","position":8,"is_corresponding":false},{"id":683160,"name":"Anna Kosmach","orcid":"0000-0002-9775-8058","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-18T23:45:22.477398Z","pmid":"34686324","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}