{"doi":"10.1016/j.celrep.2021.109727","title":"CCR2 deficiency alters activation of microglia subsets in traumatic brain injury","abstract":"In traumatic brain injury (TBI), a diversity of brain resident and peripherally derived myeloid cells have the potential to worsen damage and/or to assist in healing. We define the heterogeneity of microglia and macrophage phenotypes during TBI in wild-type (WT) mice and Ccr2 −/− mice, which lack macrophage influx following TBI and are resistant to brain damage. We use unbiased single-cell RNA sequencing methods to uncover 25 microglia, monocyte/macrophage, and dendritic cell subsets in acute TBI and normal brains. We find alterations in transcriptional profiles of microglia subsets in Ccr2 −/− TBI mice compared to WT TBI mice indicating that infiltrating monocytes/macrophages influence microglia activation to promote a type I IFN response. Preclinical pharmacological blockade of hCCR2 after injury reduces expression of IFN-responsive gene, Irf7 , and improves outcomes. These data extend our understanding of myeloid cell diversity and crosstalk in brain trauma and identify therapeutic targets in myeloid subsets.","journal":"Cell Reports","year":2021,"id":152211,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":77,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9409,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":646465,"name":"Joshua Rudolph","orcid":"0000-0002-5006-8316","position":1,"is_corresponding":false},{"id":647255,"name":"Isabella Imhof","orcid":null,"position":2,"is_corresponding":false},{"id":646466,"name":"Luyi Li","orcid":"0000-0001-6096-6403","position":3,"is_corresponding":false},{"id":2815,"name":"Eréne C. Niemi","orcid":null,"position":4,"is_corresponding":false},{"id":647256,"name":"Judy K. Shigenaga","orcid":null,"position":5,"is_corresponding":false},{"id":520833,"name":"Huy Tran","orcid":"0000-0002-9057-060X","position":6,"is_corresponding":false},{"id":242022,"name":"Thomas M. Gill","orcid":"0000-0002-6450-0368","position":7,"is_corresponding":false},{"id":646467,"name":"Iris Lo","orcid":"0000-0003-4905-9612","position":8,"is_corresponding":false},{"id":646468,"name":"Brian A. Zabel","orcid":"0000-0001-7293-6180","position":9,"is_corresponding":false},{"id":58625,"name":"Gabriela Schmajuk","orcid":"0000-0003-2687-5043","position":10,"is_corresponding":false},{"id":647257,"name":"Brian T. Wipke","orcid":null,"position":11,"is_corresponding":false},{"id":646469,"name":"Stefka Gyoneva","orcid":"0000-0001-7714-9450","position":12,"is_corresponding":false},{"id":646470,"name":"Luke Jandreski","orcid":"0000-0002-5689-1784","position":13,"is_corresponding":false},{"id":647258,"name":"Michael Craft","orcid":null,"position":14,"is_corresponding":false},{"id":647259,"name":"Gina Benedetto","orcid":null,"position":15,"is_corresponding":false},{"id":240299,"name":"Edward D. Plowey","orcid":"0000-0001-9016-4751","position":16,"is_corresponding":false},{"id":647260,"name":"Israel Charo","orcid":null,"position":17,"is_corresponding":false},{"id":646471,"name":"James J. Campbell","orcid":"0000-0003-4252-5182","position":18,"is_corresponding":false},{"id":2802,"name":"Chun Jimmie Ye","orcid":"0000-0001-6560-3783","position":19,"is_corresponding":false},{"id":647261,"name":"S. Scott Panter","orcid":null,"position":20,"is_corresponding":false},{"id":2823,"name":"Mary C. Nakamura","orcid":"0000-0001-8127-9426","position":21,"is_corresponding":false},{"id":247651,"name":"Walter L. Eckalbar","orcid":"0000-0003-2520-7852","position":22,"is_corresponding":false},{"id":28875,"name":"Christine L. Hsieh","orcid":null,"position":23,"is_corresponding":false},{"id":647254,"name":"Kerri Somebang","orcid":null,"position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-18T23:43:22.562252Z","pmid":"34551293","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}