{"doi":"10.1016/j.celrep.2021.109716","title":"Myosin-X and talin modulate integrin activity at filopodia tips","abstract":null,"journal":"Cell Reports","year":2021,"id":661364,"datarank":0.6261580904843456,"base_score":4.174387269895637,"endowment":4.174387269895637,"self_citation_contribution":0.6261580904843456,"citation_network_contribution":0.0,"self_endowment_contribution":0.6261580904843456,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1726530,"name":"Max L.B. Grönloh","orcid":null,"position":1,"is_corresponding":false},{"id":1726533,"name":"Ana Popović","orcid":null,"position":2,"is_corresponding":false},{"id":109769,"name":"Helena Vihinen","orcid":"0000-0003-3862-9237","position":3,"is_corresponding":false},{"id":109770,"name":"Eija Jokitalo","orcid":"0000-0002-4159-6934","position":4,"is_corresponding":false},{"id":73326,"name":"Benjamin T. Goult","orcid":"0000-0002-3438-2807","position":5,"is_corresponding":false},{"id":555952,"name":"Johanna Ivaska","orcid":"0000-0002-6295-6556","position":6,"is_corresponding":false},{"id":313564,"name":"Guillaume Jacquemet","orcid":"0000-0002-9286-920X","position":7,"is_corresponding":false},{"id":1559015,"name":"Mitro Miihkinen","orcid":"0000-0001-6822-3647","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Myosin-X and talin modulate integrin activity at filopodia tips","abstract":"Filopodia assemble unique integrin-adhesion complexes to sense the extracellular matrix. However, the mechanisms of integrin regulation in filopodia are poorly defined. Here, we report that active integrins accumulate at the tip of myosin-X (MYO10)-positive filopodia, while inactive integrins are uniformly distributed. We identify talin and MYO10 as the principal integrin activators in filopodia. In addition, deletion of MYO10's FERM domain, or mutation of its β1-integrin-binding residues, reveals MYO10 as facilitating integrin activation, but not transport, in filopodia. However, MYO10's isolated FERM domain alone cannot activate integrins, potentially because of binding to both integrin tails. Finally, because a chimera construct generated by swapping MYO10-FERM by talin-FERM enables integrin activation in filopodia, our data indicate that an integrin-binding FERM domain coupled to a myosin motor is a core requirement for integrin activation in filopodia. Therefore, we propose a two-step integrin activation model in filopodia: receptor tethering by MYO10 followed by talin-mediated integrin activation.","is_dataset_classified":null,"base_score":4.174387269895637,"endowment":4.174387269895637,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34525374","pmcid":"PMC8456781","openalex_id":"https://openalex.org/W3199134013","authors":[],"funders":[{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/N007336/1","title":"The molecular basis of crosstalk between integrin-mediated cell-matrix adhesions and the microtubule network."},{"funder_name":"Academy of Finland","grant_id":"325464","title":"Adhesion dynamics and mechanobiological pathways as determinants of oncogenic properties"},{"funder_name":"Academy of Finland","grant_id":"338537","title":"Filopodia-mediated communication in cancer progression (FILOCOM)"},{"funder_name":"European Research Council","grant_id":"615258","title":"Adhesion switches in cancer and development: from in vivo to synthetic biology"},{"funder_name":"Cancer Foundation Finland sr","grant_id":"200084","title":null},{"funder_name":"Cancer Foundation Finland sr","grant_id":"190114","title":null},{"funder_name":"Ida Montinin Säätiö","grant_id":"","title":null},{"funder_name":"Lounais-Suomen Syöpäyhdistys","grant_id":"","title":null},{"funder_name":"Biocenter Finland","grant_id":"","title":null},{"funder_name":"K. Albin Johanssons Stiftelse","grant_id":"","title":null},{"funder_name":"Swedish Cultural Foundation","grant_id":"","title":null},{"funder_name":"Syöpäsäätiö","grant_id":"","title":null},{"funder_name":"Maud Kuistilan Muistosäätiö","grant_id":"","title":null},{"funder_name":"Sigrid Juséliuksen Säätiö","grant_id":"","title":null},{"funder_name":"European Research Council","grant_id":"","title":null},{"funder_name":"Instrumentarium Science Foundation","grant_id":"","title":null},{"funder_name":"Syöpäjärjestöt","grant_id":"","title":null},{"funder_name":"Turun Yliopisto","grant_id":"","title":null}],"total_grants":18,"fwci":4.7623,"citation_percentile":0.96553787,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":15},{"year":2023,"count":17},{"year":2024,"count":14},{"year":2025,"count":8},{"year":2026,"count":7}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"http://www.cell.com/article/S2211124721011657/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S2211124721011657/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2211124721011657?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2211124721011657?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.celrep.2021.109716","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34525374","host_type":"repository"},{"url":"https://research.abo.fi/en/publications/c3681a9c-5267-4aae-b0c5-345264c010b0","host_type":"repository"},{"url":"https://kar.kent.ac.uk/90220/1/2021_Miihkinen_CellReps.pdf","host_type":"repository"},{"url":"https://doi.org/10.1016/j.celrep.2021.109716>)","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC8456781","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8456781","host_type":"repository"},{"url":"https://doaj.org/article/58b81ee3731645c28b9246624729fde9","host_type":"repository"},{"url":"http://hdl.handle.net/10138/335161","host_type":"journal"},{"url":"https://europepmc.org/articles/PMC8456781","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1016/j.celrep.2021.109716","host_type":""},{"url":"http://juuli.fi/Record/0382033921","host_type":""},{"url":"https://kar.kent.ac.uk/81141/13/1-s2.0-S2211124721011657-main.pdf","host_type":""},{"url":"https://dx.doi.org/10.1016/j.celrep.2021.109716","host_type":""}],"fields_of_study":["Cellular Mechanics and Interactions","Cell Adhesion Molecules Research","Cellular transport and secretion","0301 basic medicine","03 medical and health sciences","0303 health sciences","Binding Sites","Cell Line, Tumor","Focal Adhesions","Humans","Integrin beta1","Myosins","Protein Binding","Protein Domains","Pseudopodia","RNA Interference","RNA, Small Interfering","Talin"],"mesh_terms":["Protein Domains","Binding Sites","Humans","Myosins","Protein Binding","Pseudopodia","Talin","Integrin beta1","Focal Adhesions","RNA Interference","RNA, Small Interfering","Cell Line, Tumor"],"keywords":["Filopodia","FERM domain","Integrin","Cell biology","Myosin","Biology","Chemistry","Actin","Receptor","Biochemistry","Membrane protein","adhesion","Talin","Myo10","Integrin Activity","MECHANISM","DYNAMICS","QH301-705.5","CARGO RECOGNITION","Myosins","UP-REGULATION","ta3111","Article","ACTIVATION","Protein Domains","Cell Line, Tumor","REVEALS","Humans","ta318","Pseudopodia","Biology (General)","RNA, Small Interfering","Biochemistry, cell and molecular biology","QH581.2","Focal Adhesions","COMPLEX","Binding Sites","ELONGATION","Integrin beta1","ta1182","CONFORMATION","RNA Interference","CELL-ADHESION","Protein Binding"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T10:52:02.216940Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}