{"doi":"10.1016/j.celrep.2021.109269","title":"Transcriptional repression by FEZF2 restricts alternative identities of cortical projection neurons","abstract":"Projection neuron subtype identities in the cerebral cortex are established by expressing pan-cortical and subtype-specific effector genes that execute terminal differentiation programs bestowing neurons with a glutamatergic neuron phenotype and subtype-specific morphology, physiology, and axonal projections. Whether pan-cortical glutamatergic and subtype-specific characteristics are regulated by the same genes or controlled by distinct programs remains largely unknown. Here, we show that FEZF2 functions as a transcriptional repressor, and it regulates subtype-specific identities of both corticothalamic and subcerebral neurons by selectively repressing expression of genes inappropriate for each neuronal subtype. We report that TLE4, specifically expressed in layer 6 corticothalamic neurons, is recruited by FEZF2 to inhibit layer 5 subcerebral neuronal genes. Together with previous studies, our results indicate that a cortical glutamatergic identity is specified by multiple parallel pathways active in progenitor cells, whereas projection neuron subtype-specific identity is achieved through selectively repressing genes associated with alternate identities in differentiating neurons.","journal":"Cell Reports","year":2021,"id":156744,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":53,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":662075,"name":"David Tastad","orcid":"0000-0002-6378-8275","position":1,"is_corresponding":false},{"id":662076,"name":"Xiaokuang Ma","orcid":"0000-0001-7900-6779","position":2,"is_corresponding":false},{"id":662077,"name":"Antoine Nehme","orcid":"0000-0002-6729-2445","position":3,"is_corresponding":false},{"id":468881,"name":"Thomas S. Finn","orcid":"0000-0002-4522-5321","position":4,"is_corresponding":false},{"id":662735,"name":"Liora Huebner","orcid":null,"position":5,"is_corresponding":false},{"id":285839,"name":"Guoping Liu","orcid":"0000-0002-2352-1138","position":6,"is_corresponding":false},{"id":662078,"name":"Daisy Gallardo","orcid":"0000-0002-6424-2020","position":7,"is_corresponding":false},{"id":662079,"name":"Amr Makhamreh","orcid":"0000-0002-4615-5820","position":8,"is_corresponding":false},{"id":662080,"name":"Jacqueline M. Roberts","orcid":"0000-0002-1024-3976","position":9,"is_corresponding":false},{"id":662736,"name":"Solomon Katzman","orcid":null,"position":10,"is_corresponding":false},{"id":21538,"name":"Nenad Šestan","orcid":"0000-0003-0966-9619","position":11,"is_corresponding":false},{"id":662081,"name":"Susan K. McConnell","orcid":"0000-0003-0668-9569","position":12,"is_corresponding":false},{"id":285847,"name":"Zhengang Yang","orcid":"0000-0003-2447-6540","position":13,"is_corresponding":false},{"id":447130,"name":"Shenfeng Qiu","orcid":"0000-0002-0730-1602","position":14,"is_corresponding":false},{"id":662082,"name":"Bin Chen","orcid":"0000-0002-5227-7736","position":15,"is_corresponding":false},{"id":285846,"name":"Jeremiah Tsyporin","orcid":"0000-0001-6588-6840","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-18T23:44:13.017332Z","pmid":"34161768","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}