{"doi":"10.1016/j.celrep.2021.109039","title":"The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis","abstract":"The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study this process of the type II NB lineage. Combining prior knowledge, in silico analyses, and in situ validation, our multi-informatic investigation describes the molecular landscape from a single developmental snapshot. 17 markers are identified to differentiate distinct maturation stages. 30 markers are identified to specify the stem cell origin and/or cell division numbers of INPs, and at least 12 neuronal subtypes are identified. To foster future discoveries, we provide annotated tables of pairwise gene-gene correlation in single cells and MiCV, a web tool for interactively analyzing scRNA-seq datasets. Taken together, these resources advance our understanding of the neural differentiation process at the molecular level.","journal":"Cell Reports","year":2021,"id":164178,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9115,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":557750,"name":"Ye Li","orcid":"0000-0002-8647-384X","position":1,"is_corresponding":false},{"id":586374,"name":"Kayvon Sanjasaz","orcid":null,"position":2,"is_corresponding":false},{"id":557751,"name":"Yimeng Zhao","orcid":"0000-0002-4217-7061","position":3,"is_corresponding":false},{"id":321069,"name":"Fred Shen","orcid":"0000-0002-2345-780X","position":4,"is_corresponding":false},{"id":321070,"name":"Logan A. Walker","orcid":"0000-0002-5378-3315","position":5,"is_corresponding":false},{"id":547861,"name":"Wenjia Cao","orcid":null,"position":6,"is_corresponding":false},{"id":573580,"name":"Cheng-Yu Lee","orcid":"0000-0003-2291-1297","position":7,"is_corresponding":false},{"id":306032,"name":"Dawen Cai","orcid":"0000-0003-4471-2061","position":8,"is_corresponding":false},{"id":557752,"name":"Nigel S. Michki","orcid":"0000-0003-0403-0648","position":0,"is_corresponding":true}],"reference_count":84,"raw_metadata":null,"created_at":"2026-07-18T23:45:27.031465Z","pmid":"33909998","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}