{"doi":"10.1016/j.celrep.2021.108974","title":"High-dimensional profiling clusters asthma severity by lymphoid and non-lymphoid status","abstract":"Clinical definitions of asthma fail to capture the heterogeneity of immune dysfunction in severe, treatment-refractory disease. Applying mass cytometry and machine learning to bronchoalveolar lavage (BAL) cells, we find that corticosteroid-resistant asthma patients cluster largely into two groups: one enriched in interleukin (IL)-4+ innate immune cells and another dominated by interferon (IFN)-γ+ T cells, including tissue-resident memory cells. In contrast, BAL cells of a healthier population are enriched in IL-10+ macrophages. To better understand cellular mediators of severe asthma, we developed the Immune Cell Linkage through Exploratory Matrices (ICLite) algorithm to perform deconvolution of bulk RNA sequencing of mixed-cell populations. Signatures of mitosis and IL-7 signaling in CD206−FcεRI+CD127+IL-4+ innate cells in one patient group, contrasting with adaptive immune response in T cells in the other, are preserved across technologies. Transcriptional signatures uncovered by ICLite identify T-cell-high and T-cell-poor severe asthma patients in an independent cohort, suggesting broad applicability of our findings.","journal":"Cell Reports","year":2021,"id":154645,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":62,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9516,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":524239,"name":"Xiaoying Zhou","orcid":"0000-0002-3177-3271","position":1,"is_corresponding":false},{"id":655194,"name":"Timothy B. Oriss","orcid":"0000-0002-9725-9996","position":2,"is_corresponding":false},{"id":263117,"name":"Qi Yan","orcid":"0000-0002-5236-9673","position":3,"is_corresponding":false},{"id":655195,"name":"Michael C. Gorry","orcid":"0000-0001-8370-7754","position":4,"is_corresponding":false},{"id":501140,"name":"William Horne","orcid":"0000-0003-3933-7404","position":5,"is_corresponding":false},{"id":289433,"name":"John B. Trudeau","orcid":"0000-0002-0473-2730","position":6,"is_corresponding":false},{"id":656077,"name":"Kathryn Scholl","orcid":null,"position":7,"is_corresponding":false},{"id":109275,"name":"Wei Chen","orcid":"0000-0001-7196-8703","position":8,"is_corresponding":false},{"id":246970,"name":"Jay K. Kolls","orcid":"0000-0001-5151-6304","position":9,"is_corresponding":false},{"id":281864,"name":"Prabir Ray","orcid":"0000-0002-0430-5114","position":10,"is_corresponding":false},{"id":237067,"name":"Florian Weisel","orcid":"0000-0002-8679-9241","position":11,"is_corresponding":false},{"id":656078,"name":"Nadine Weisel","orcid":null,"position":12,"is_corresponding":false},{"id":226949,"name":"Nima Aghaeepour","orcid":"0000-0002-6117-8764","position":13,"is_corresponding":false},{"id":36438,"name":"Kari C. Nadeau","orcid":"0000-0002-2146-2955","position":14,"is_corresponding":false},{"id":218543,"name":"Sally E. Wenzel","orcid":"0000-0002-4242-0164","position":15,"is_corresponding":false},{"id":269706,"name":"Anuradha Ray","orcid":"0000-0001-6844-8918","position":16,"is_corresponding":false},{"id":269704,"name":"Matthew Camiolo","orcid":"0000-0002-9085-6552","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-18T23:43:54.469024Z","pmid":"33852838","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}