{"doi":"10.1016/j.celrep.2020.108320","title":"In Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells","abstract":"We report a serum-free, 3D murine artificial thymic organoid (M-ATO) system that mimics normal murine thymopoiesis with the production of all T cell stages, from early thymic progenitors to functional single-positive (CD8SP and CD4SP) TCRαβ and TCRγδ cells. RNA sequencing aligns M-ATO-derived populations with phenotypically identical primary thymocytes. M-ATOs initiated with Rag1 −/− marrow produce the same differentiation block as seen in the endogenous thymus, and Notch signaling patterns in M-ATOs mirror primary thymopoiesis. M-ATOs initiated with defined hematopoietic stem cells (HSCs) and lymphoid progenitors from marrow and thymus generate each of the downstream differentiation stages, allowing the kinetics of T cell differentiation to be tracked. Remarkably, single HSCs deposited into each M-ATO generate the complete trajectory of T cell differentiation, producing diverse TCR repertoires across clones that largely match endogenous thymus. M-ATOs represent a highly reproducible and efficient experimental platform for the interrogation of clonal thymopoiesis from HSCs.","journal":"Cell Reports","year":2020,"id":93040,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.953,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":466382,"name":"Victoria Sun","orcid":null,"position":1,"is_corresponding":false},{"id":36321,"name":"David Casero","orcid":"0000-0002-7347-3330","position":2,"is_corresponding":false},{"id":465137,"name":"Steven Tsai","orcid":"0009-0007-6329-7787","position":3,"is_corresponding":false},{"id":466383,"name":"Alexandre Zampieri","orcid":null,"position":4,"is_corresponding":false},{"id":465138,"name":"Nicholas Jackson","orcid":"0000-0003-4837-6624","position":5,"is_corresponding":false},{"id":465139,"name":"Suwen Li","orcid":"0000-0002-2296-6389","position":6,"is_corresponding":false},{"id":466384,"name":"Shawn Lopez","orcid":null,"position":7,"is_corresponding":false},{"id":465140,"name":"Yuhua Zhu","orcid":"0000-0002-7237-9828","position":8,"is_corresponding":false},{"id":84645,"name":"Brent Chick","orcid":"0000-0002-0193-1654","position":9,"is_corresponding":false},{"id":466385,"name":"Chongbin He","orcid":null,"position":10,"is_corresponding":false},{"id":465141,"name":"Stéphanie C. de Barros","orcid":"0000-0003-1168-069X","position":11,"is_corresponding":false},{"id":281852,"name":"Christopher S. Seet","orcid":"0000-0002-4821-1400","position":12,"is_corresponding":false},{"id":281853,"name":"Gay M. Crooks","orcid":"0000-0002-2488-8468","position":13,"is_corresponding":false},{"id":283568,"name":"Amélie Montel‐Hagen","orcid":null,"position":0,"is_corresponding":true}],"reference_count":63,"raw_metadata":null,"created_at":"2026-07-18T22:30:54.208860Z","pmid":"33113379","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}