{"doi":"10.1016/j.celrep.2020.108187","title":"Heterogeneity in VEGF Receptor-2 Mobility and Organization on the Endothelial Cell Surface Leads to Diverse Models of Activation by VEGF","abstract":"The dynamic nanoscale organization of cell surface receptors plays an important role in signaling. We determine this organization and its relation to activation of VEGF receptor-2 (VEGFR-2), a critical receptor tyrosine kinase in endothelial cells (ECs), by combining single-molecule imaging of endogenous VEGFR-2 in live ECs with multiscale computational analysis. We find that surface VEGFR-2 can be mobile or exhibit restricted mobility and be monomeric or non-monomeric, with a complex interplay between the two. This basal heterogeneity results in heterogeneity in the sequence of steps leading to VEGFR-2 activation by VEGF. Specifically, we find that VEGF can bind to monomeric and non-monomeric VEGFR-2 and that, when binding to monomeric VEGFR-2, its effect on dimerization depends on the mobility of VEGFR-2. Our study highlights the dynamic and heterogeneous nature of cell surface receptor organization and the need for multiscale, single-molecule-based analysis to determine its relationship to receptor activation and signaling.","journal":"Cell Reports","year":2020,"id":91528,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":45,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":458598,"name":"Sungsoo Lee","orcid":"0000-0001-8998-9510","position":1,"is_corresponding":false},{"id":458599,"name":"Aparajita Dasgupta","orcid":"0000-0001-9843-9879","position":2,"is_corresponding":false},{"id":458600,"name":"Anthony R. Vega","orcid":"0000-0002-4464-6482","position":3,"is_corresponding":false},{"id":458601,"name":"Luciana R. de Oliveira","orcid":"0000-0002-0546-4416","position":4,"is_corresponding":false},{"id":458602,"name":"Tae Il Kim","orcid":"0000-0003-4807-890X","position":5,"is_corresponding":false},{"id":44548,"name":"Mark Kittisopikul","orcid":"0000-0002-9558-6248","position":6,"is_corresponding":false},{"id":460073,"name":"Zachariah Malik","orcid":null,"position":7,"is_corresponding":false},{"id":451244,"name":"Khuloud Jaqaman","orcid":"0000-0003-3471-1911","position":8,"is_corresponding":false},{"id":458597,"name":"Bruno da Rocha-Azevedo","orcid":"0000-0002-4684-161X","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-18T22:29:21.932251Z","pmid":"32997988","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}