{"doi":"10.1016/j.celrep.2020.108003","title":"Differential Histone Distribution Patterns in Induced Asymmetrically Dividing Mouse Embryonic Stem Cells","abstract":"Wnt3a-coated beads can induce asymmetric divisions of mouse embryonic stem cells (mESCs), resulting in one self-renewed mESC and one differentiating epiblast stem cell. This provides an opportunity for studying histone inheritance pattern at a single-cell resolution in cell culture. Here, we report that mESCs with Wnt3a-bead induction display nonoverlapping preexisting (old) versus newly synthesized (new) histone H3 patterns, but mESCs without Wnt3a beads have largely overlapping patterns. Furthermore, H4K20me2/3, an old histone-enriched modification, displays a higher instance of asymmetric distribution on chromatin fibers from Wnt3a-induced mESCs than those from non-induced mESCs. These locally distinct distributions between old and new histones have both cellular specificity in Wnt3a-induced mESCs and molecular specificity for histones H3 and H4. Given that post-translational modifications at H3 and H4 carry the major histone modifications, our findings provide a mammalian cell culture system to study histone inheritance for maintaining stem cell fate and for resetting it during differentiation.","journal":"Cell Reports","year":2020,"id":91844,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":391992,"name":"Tung-Jui Trieu","orcid":"0000-0002-9457-4173","position":1,"is_corresponding":false},{"id":459591,"name":"Ji Cheng","orcid":"0000-0003-1610-2557","position":2,"is_corresponding":false},{"id":459592,"name":"Shuang Zhou","orcid":"0000-0002-6993-8617","position":3,"is_corresponding":false},{"id":459593,"name":"Qingsong Tang","orcid":"0000-0002-9894-870X","position":4,"is_corresponding":false},{"id":459594,"name":"Jing Xie","orcid":"0000-0001-5624-458X","position":5,"is_corresponding":false},{"id":459595,"name":"Ji‐Long Liu","orcid":"0000-0002-4834-8554","position":6,"is_corresponding":false},{"id":233091,"name":"Keji Zhao","orcid":"0000-0001-5559-6233","position":7,"is_corresponding":false},{"id":391993,"name":"Shukry J. Habib","orcid":"0000-0003-3132-2216","position":8,"is_corresponding":false},{"id":459596,"name":"Xin Chen","orcid":"0000-0002-5189-5647","position":9,"is_corresponding":false},{"id":391991,"name":"Bin Ma","orcid":"0009-0003-1048-4459","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-18T22:29:21.932251Z","pmid":"32783931","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}