{"doi":"10.1016/j.celrep.2020.107849","title":"The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells","abstract":"Replication-blocking DNA lesions are particularly toxic to proliferating cells because they can lead to chromosome mis-segregation if not repaired prior to mitosis. In this study, we report that ZGRF1 null cells accumulate chromosome aberrations following replication perturbation and show sensitivity to two potent replication-blocking anticancer drugs: mitomycin C and camptothecin. Moreover, ZGRF1 null cells are defective in catalyzing DNA damage-induced sister chromatid exchange despite accumulating excessive FANCD2, RAD51, and γ-H2AX foci upon induction of interstrand DNA crosslinks. Consistent with a direct role in promoting recombinational DNA repair, we show that ZGRF1 is a 5'-to-3' helicase that catalyzes D-loop dissociation and Holliday junction branch migration. Moreover, ZGRF1 physically interacts with RAD51 and stimulates strand exchange catalyzed by RAD51-RAD54. On the basis of these data, we propose that ZGRF1 promotes repair of replication-blocking DNA lesions through stimulation of homologous recombination.","journal":"Cell Reports","year":2020,"id":72091,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":380566,"name":"Xiaoyu Xue","orcid":"0000-0001-9654-6566","position":1,"is_corresponding":false},{"id":278335,"name":"Youngho Kwon","orcid":"0000-0002-0287-8795","position":2,"is_corresponding":false},{"id":382020,"name":"Smaragdi Kompocholi","orcid":null,"position":3,"is_corresponding":false},{"id":232590,"name":"Anne Simonsen","orcid":"0000-0003-4711-7057","position":4,"is_corresponding":false},{"id":380567,"name":"Keerthana Stine Viswalingam","orcid":"0000-0002-2179-1804","position":5,"is_corresponding":false},{"id":380568,"name":"Leticia Gonzalez","orcid":"0000-0003-1057-1096","position":6,"is_corresponding":false},{"id":247629,"name":"Ian D. Hickson","orcid":"0000-0002-0583-566X","position":7,"is_corresponding":false},{"id":380569,"name":"Vibe H. Oestergaard","orcid":"0000-0002-1633-0560","position":8,"is_corresponding":false},{"id":380570,"name":"Hocine W Mankouri","orcid":"0000-0003-2092-0907","position":9,"is_corresponding":false},{"id":278336,"name":"Patrick Sung","orcid":"0000-0003-1396-9040","position":10,"is_corresponding":false},{"id":380571,"name":"Michael Lisby","orcid":"0000-0002-4830-5247","position":11,"is_corresponding":false},{"id":382019,"name":"André Brannvoll","orcid":null,"position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-18T21:44:45.622639Z","pmid":"32640219","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}